DGCR14 sirna

DGCR14 siRNA (Mouse)

Cat. Num. AAA8212717

• Gene Names: Dgcr14; ES2; Dgsi; Dgcr1; Es2el; AI462402; D16H22S1269E
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DGCR14; DGCR14 siRNA (Mouse); DGSI; ES2; ES2EL; Protein DGCR14; DiGeorge syndrome critical region 14 homolog; ES2 protein; Expressed sequence 2 embryonic lethal; DGCR14 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: DGCR14 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 479

• Applicable Applications for DGCR14 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DGCR14 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DGCR14 sirna

siRNA to inhibit DGCR14 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 126362981
• NCBI GeneID: 27886
• NCBI Accession #: NP_001075102.1
• NCBI GenBank Nucleotide #: NM_001081633.1
• UniProt Accession #: O70279; Q91YX1
• Molecular Weight: 52,604 Da
• NCBI Official Full Name: protein DGCR14 isoform 2
• NCBI Official Synonym Full Names: DiGeorge syndrome critical region gene 14
• NCBI Official Symbol: Dgcr14
• NCBI Official Synonym Symbols: ES2; Dgsi; Dgcr1; Es2el; AI462402; D16H22S1269E
• NCBI Protein Information: protein DGCR14
• UniProt Protein Name: Protein DGCR14
• Protein Family: Protein
• UniProt Gene Name: Dgcr14
• UniProt Synonym Gene Names: Dgsi; Es2; Es2el
• UniProt Entry Name: DGC14_MOUSE

NCBI Description

The human ortholog of this gene is located within the minimal DGS critical region (MDGCR) thought to contain the gene(s) responsible for a group of developmental disorders. These disorders include DiGeorge syndrome, velocardiofacial syndrome, conotruncal anomaly face syndrome, and some familial or sporadic conotruncal cardiac defects which have been associated with microdeletion of human chromosome band 22q11.2. The encoded protein localizes to the nucleus, and the orthologous protein in humans co-purifies with C complex spliceosomes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

DGCR14: involved in pre-mRNA splicing. One of the many proteins in the spliceosome C complex. Defects in this gene play a part in the etiology of the velocardiofacial/DiGeorge syndrome (VCFS/DGS).

Protein type: Spliceosome; RNA-binding; RNA splicing; RNA processing

Cellular Component: spliceosome; nucleus

Biological Process: RNA splicing; mRNA processing