DEGS2 sirna

DEGS2 siRNA (Mouse)

Cat. Num. AAA8202455

• Gene Names: Degs2; DES2; AI852933; 2210008A03Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DEGS2; DEGS2 siRNA (Mouse); Sphingolipid delta(4)-desaturase/C4-hydroxylase DES2; Degenerative spermatocyte homolog 2; DEGS2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: DEGS2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 286

• Applicable Applications for DEGS2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DEGS2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DEGS2 sirna

siRNA to inhibit DEGS2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 283483988
• NCBI GeneID: 70059
• NCBI Accession #: NP_001164473.1
• NCBI GenBank Nucleotide #: NM_001171002.1
• UniProt Accession #: Q8R2F2; Q3TR85; Q78JJ1
• Molecular Weight: 33,020 Da
• NCBI Official Full Name: sphingolipid delta(4)-desaturase/C4-hydroxylase DES2 isoform 2
• NCBI Official Synonym Full Names: degenerative spermatocyte homolog 2 (Drosophila), lipid desaturase
• NCBI Official Symbol: Degs2
• NCBI Official Synonym Symbols: DES2; AI852933; 2210008A03Rik
• NCBI Protein Information: sphingolipid delta(4)-desaturase/C4-hydroxylase DES2
• UniProt Protein Name: Sphingolipid delta(4)-desaturase/C4-hydroxylase DES2
• UniProt Gene Name: Degs2
• UniProt Entry Name: DEGS2_MOUSE

Uniprot Description

DEGS2: Bifunctional enzyme which acts as both a sphingolipid delta(4)-desaturase and a sphingolipid C4-hydroxylase. Belongs to the fatty acid desaturase family. DEGS subfamily.

Protein type: Membrane protein, multi-pass; EC 1.-.-.-; Oxidoreductase; Lipid Metabolism - sphingolipid; Membrane protein, integral; EC 1.14.-.-

Cellular Component: membrane; endoplasmic reticulum; integral to membrane

Molecular Function: tert-butyl alcohol 2-monooxygenase activity; N-ethylmaleimide reductase activity; methylsilanetriol hydroxylase activity; sphingosine hydroxylase activity; alpha-pinene dehydrogenase activity; hydroxymethylsilanetriol oxidase activity; oxidoreductase activity; tri-n-butyltin dioxygenase activity; fluorene oxygenase activity; sulfur oxygenase reductase activity; 2-hydroxyisobutyrate 3-monooxygenase activity; 4-nitrocatechol 4-monooxygenase activity; alpha-pinene monooxygenase activity; spheroidene monooxygenase activity; dimethylsilanediol hydroxylase activity; malolactic enzyme activity; mono-butyltin dioxygenase activity; ammonia monooxygenase activity; 4-chlorophenoxyacetate monooxygenase activity; sphingolipid delta-4 desaturase activity; methyl tertiary butyl ether 3-monooxygenase activity; di-n-butyltin dioxygenase activity; F420H2 dehydrogenase activity

Biological Process: sphingolipid biosynthetic process; ceramide biosynthetic process; lipid metabolic process; sphinganine metabolic process