DDIT3 sirna

DDIT3 siRNA (Mouse)

Cat. Num. AAA8212782

• Gene Names: Ddit3; chop; CHOP10; CHOP-10; gadd153
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DDIT3; DDIT3 siRNA (Mouse); CHOP; CHOP10; GADD153; DNA damage-inducible transcript 3 protein; DDIT-3; C/EBP zeta; C/EBP-homologous protein; C/EBP-homologous protein 10; CHOP-10; CCAAT/enhancer-binding protein homologous protein; Growth arrest and DNA-damage-inducible protein GADD153; DDIT3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: DDIT3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 168

• Applicable Applications for DDIT3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DDIT3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DDIT3 sirna

siRNA to inhibit DDIT3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 589058168
• NCBI GeneID: 13198
• NCBI Accession #: NP_001277112.1
• NCBI GenBank Nucleotide #: NM_001290183.1
• UniProt Accession #: P35639; Q91YW9
• Molecular Weight: 19,189 Da
• NCBI Official Full Name: DNA damage-inducible transcript 3 protein
• NCBI Official Synonym Full Names: DNA-damage inducible transcript 3
• NCBI Official Symbol: Ddit3
• NCBI Official Synonym Symbols: chop; CHOP10; CHOP-10; gadd153
• NCBI Protein Information: DNA damage-inducible transcript 3 protein
• UniProt Protein Name: DNA damage-inducible transcript 3 protein
• Protein Family: DNA damage-inducible transcript 3 protein
• UniProt Gene Name: Ddit3
• UniProt Synonym Gene Names: Chop; Chop10; Gadd153; DDIT-3; CHOP; CHOP-10
• UniProt Entry Name: DDIT3_MOUSE

Uniprot Description

CHOP: a transcriptional-regulatory protein of the bZIP family. Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA. May play an important role in melanoma progression. CK2-mediated phosphorylation inhibits its transcriptional activity. Up-regulates IL-6 transcription by trapping negative regulating NF-IL6 isoform.

Protein type: DNA-binding; Autophagy; Transcription factor; Oncoprotein

Cellular Component: nucleoplasm; cell; late endosome; cytoplasm; intracellular; cytosol; nucleus

Molecular Function: protein binding; leucine zipper domain binding; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; transcription factor activity; transcription factor binding

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; positive regulation of apoptosis; apoptosis; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; positive regulation of interleukin-8 production; regulation of transcription, DNA-dependent; positive regulation of neuron apoptosis; cell cycle arrest; ER overload response; negative regulation of protein kinase B signaling cascade; Wnt receptor signaling pathway; transcription, DNA-dependent; unfolded protein response; negative regulation of transcription factor activity; negative regulation of fat cell differentiation; cell cycle; mRNA transcription from RNA polymerase II promoter; blood vessel maturation; response to unfolded protein; negative regulation of DNA binding; response to starvation; cell redox homeostasis; release of sequestered calcium ion into cytosol; positive regulation of transcription from RNA polymerase II promoter; inhibition of CREB transcription factor; negative regulation of transcription, DNA-dependent; negative regulation of myoblast differentiation