DAXX sirna

DAXX siRNA (Mouse)

Cat. Num. AAA8235090

• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: DAXX; DAXX siRNA (Mouse); Death domain-associated protein 6; Daxx; DAXX sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: DAXX siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 740

• Applicable Applications for DAXX sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DAXX gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for DAXX sirna

siRNA to inhibit DAXX expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 315013583
• NCBI GeneID: 13163
• NCBI Accession #: NP_001186662.1
• NCBI GenBank Nucleotide #: NM_001199733.1
• UniProt Accession #: O35613; Q9QWT8; Q9QWV3
• Molecular Weight: 81,489 Da
• NCBI Official Full Name: death domain-associated protein 6
• NCBI Official Synonym Full Names: Fas death domain-associated protein
• NCBI Official Symbol: Daxx
• NCBI Protein Information: death domain-associated protein 6
• UniProt Protein Name: Death domain-associated protein 6
• Protein Family: Death domain-associated protein
• UniProt Gene Name: Daxx
• UniProt Entry Name: DAXX_MOUSE

Uniprot Description

DAXX: a transcriptional co-regulatory protein. Proposed to mediate activation of the JNK pathway and apoptosis via ASK1 in response to signaling from FAS and TGF-betaR2. Glucose deprivation activates the ASK1-SEK1-JNK1-HIPK1 pathway, relocalizing Daxx from the nucleus to the cytoplasm, where Daxx binds to ASK1, and subsequently leads to ASK1 oligomerization. Interaction with HSP27 may prevent interaction with TGF-betaR2 and ASK1 and block DAXX-mediated apoptosis. Seems to act as a transcriptional co- repressor and inhibits PAX3 and ETS1 through direct protein- protein interaction. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively. Two alternatively spliced isoforms have been described.

Protein type: Nuclear receptor co-regulator; Nucleolus; Apoptosis; Transcription, coactivator/corepressor

Cellular Component: XY body; heterochromatin; microtubule; PML body; neuron projection; cytoplasm; cell cortex; cytosol; nucleus

Molecular Function: protein binding; protein homodimerization activity; enzyme binding; androgen receptor binding; histone binding; p53 binding; ubiquitin protein ligase binding; transcription coactivator activity; protein N-terminus binding; transcription factor binding; protein kinase activator activity; protein kinase binding; transcription corepressor activity

Biological Process: transcription, DNA-dependent; positive regulation of histone phosphorylation; regulation of protein ubiquitination; apoptosis; positive regulation of apoptosis; PML body organization and biogenesis; negative regulation of transcription from RNA polymerase II promoter; chromatin modification; chromatin remodeling; nucleosome assembly; regulation of transcription, DNA-dependent; cytokinesis after mitosis; positive regulation of protein kinase activity; androgen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; negative regulation of transcription, DNA-dependent