CYP51A1 sirna

CYP51A1 siRNA (Mouse)

Cat. Num. AAA8229842

• Gene Names: Cyp51; Ldm; CYPLI; P450LI; Cyp51a1; AI426508; P450-14DM
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CYP51A1; CYP51A1 siRNA (Mouse); CYP51; Lanosterol 14-alpha demethylase; LDM; CYPLI; Cytochrome P450 51A1; Cytochrome P450-14DM; Cytochrome P45014DM; Cytochrome P450LI; Sterol 14-alpha demethylase; CYP51A1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CYP51A1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 503

• Applicable Applications for CYP51A1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CYP51A1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CYP51A1 sirna

siRNA to inhibit CYP51A1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 71061451
• NCBI GeneID: 13121
• NCBI Accession #: NP_064394.2
• NCBI GenBank Nucleotide #: NM_020010.2
• UniProt Accession #: Q8K0C4; Q8BSQ7; Q9JIP8; Q9JIY3
• Molecular Weight: 56,776 Da
• NCBI Official Full Name: lanosterol 14-alpha demethylase
• NCBI Official Synonym Full Names: cytochrome P450, family 51
• NCBI Official Symbol: Cyp51
• NCBI Official Synonym Symbols: Ldm; CYPLI; P450LI; Cyp51a1; AI426508; P450-14DM
• NCBI Protein Information: lanosterol 14-alpha demethylase
• UniProt Protein Name: Lanosterol 14-alpha demethylase
• UniProt Gene Name: Cyp51a1
• UniProt Synonym Gene Names: Cyp51; LDM; Cytochrome P45014DM
• UniProt Entry Name: CP51A_MOUSE

Uniprot Description

CYP51A1: Catalyzes C14-demethylation of lanosterol; it transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol. Belongs to the cytochrome P450 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Oxidoreductase; Lipid Metabolism - steroid biosynthesis; EC 1.14.13.70

Cellular Component: intracellular membrane-bound organelle; membrane; endoplasmic reticulum; integral to membrane

Molecular Function: sterol 14-demethylase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; metal ion binding; iron ion binding; heme binding; oxidoreductase activity; monooxygenase activity

Biological Process: steroid metabolic process; cholesterol metabolic process; sterol biosynthetic process; lipid metabolic process; steroid biosynthetic process; cholesterol biosynthetic process; cholesterol biosynthetic process via 24,25-dihydrolanosterol