CYP2A4 sirna

CYP2A4 siRNA (Mouse)

Cat. Num. AAA8234089

• Gene Names: Cyp2a4; Cyp15a1; D7Ucla4; AI893559
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CYP2A4; CYP2A4 siRNA (Mouse); CYP2A-4; Cytochrome P450 2A4; CYPIIA4; Cytochrome P450-15-alpha; Cytochrome P450-IIA3.1; Testosterone 15-alpha-hydroxylase; CYP2A4 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CYP2A4 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 494

• Applicable Applications for CYP2A4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CYP2A4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CYP2A4 sirna

siRNA to inhibit CYP2A4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 116268118
• NCBI GeneID: 13086
• NCBI Accession #: NP_034127.2
• NCBI GenBank Nucleotide #: NM_009997.2
• UniProt Accession #: P15392; Q91XG2
• Molecular Weight: 56,782 Da
• NCBI Official Full Name: cytochrome P450 2A4
• NCBI Official Synonym Full Names: cytochrome P450, family 2, subfamily a, polypeptide 4
• NCBI Official Symbol: Cyp2a4
• NCBI Official Synonym Symbols: Cyp15a1; D7Ucla4; AI893559
• NCBI Protein Information: cytochrome P450 2A4
• UniProt Protein Name: Cytochrome P450 2A4
• Protein Family: Cytochrome
• UniProt Gene Name: Cyp2a4
• UniProt Synonym Gene Names: Cyp2a-4
• UniProt Entry Name: CP2A4_MOUSE

Uniprot Description

CYP2A7: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Belongs to the cytochrome P450 family.

Protein type: Xenobiotic Metabolism - drug metabolism - other enzymes; Oxidoreductase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Cofactor and Vitamin Metabolism - retinol; EC 1.14.14.1; Endoplasmic reticulum; Secondary Metabolites Metabolism - caffeine

Cellular Component: intracellular membrane-bound organelle; cytoplasmic microtubule; cytoplasm

Molecular Function: coumarin 7-hydroxylase activity; enzyme binding; heme binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; arachidonic acid epoxygenase activity; steroid hydroxylase activity; oxygen binding

Biological Process: coumarin metabolic process; xenobiotic metabolic process; exogenous drug catabolic process; epoxygenase P450 pathway