CYP17A1 sirna

CYP17A1 siRNA (Mouse)

Cat. Num. AAA8203583

• Gene Names: Cyp17a1; Cyp17; p450c17
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CYP17A1; CYP17A1 siRNA (Mouse); CYP17; Steroid 17-alpha-hydroxylase/17.20 lyase; 17-alpha-hydroxyprogesterone aldolase; CYPXVII; Cytochrome P450 17A1; Cytochrome P450-C17; Cytochrome P450c17; CYP17A1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CYP17A1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 507

• Applicable Applications for CYP17A1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CYP17A1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CYP17A1 sirna

siRNA to inhibit CYP17A1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 160948601
• NCBI GeneID: 13074
• NCBI Accession #: NP_031835.3
• NCBI GenBank Nucleotide #: NM_007809.3
• UniProt Accession #: P27786
• Molecular Weight: 57,638 Da
• NCBI Official Full Name: steroid 17-alpha-hydroxylase/17,20 lyase
• NCBI Official Synonym Full Names: cytochrome P450, family 17, subfamily a, polypeptide 1
• NCBI Official Symbol: Cyp17a1
• NCBI Official Synonym Symbols: Cyp17; p450c17
• NCBI Protein Information: steroid 17-alpha-hydroxylase/17,20 lyase
• UniProt Protein Name: Steroid 17-alpha-hydroxylase/17,20 lyase
• Protein Family: Steroid 17-alpha-hydroxylase/17,20 lyase
• UniProt Gene Name: Cyp17a1
• UniProt Synonym Gene Names: Cyp17; Cytochrome P450c17
• UniProt Entry Name: CP17A_MOUSE

Uniprot Description

CYP17A1: Conversion of pregnenolone and progesterone to their 17- alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty. Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5). AH5 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: salt wasting (SW, the most severe type), simple virilizing (SV, less severely affected patients), with normal aldosterone biosynthesis, non-classic form or late onset (NC or LOAH), and cryptic (asymptomatic). Belongs to the cytochrome P450 family.

Protein type: Oxidoreductase; Lipid Metabolism - C21-steroid hormone; EC 1.14.99.9; EC 4.1.2.30

Cellular Component: cell projection; mitochondrion; intracellular membrane-bound organelle; membrane; cell soma; endoplasmic reticulum; axon

Molecular Function: 17-alpha-hydroxyprogesterone aldolase activity; steroid 17-alpha-monooxygenase activity; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; metal ion binding; iron ion binding; lyase activity; heme binding; oxidoreductase activity; monooxygenase activity

Biological Process: steroid metabolic process; hormone biosynthetic process; progesterone metabolic process; glucocorticoid biosynthetic process; steroid biosynthetic process