CTSH sirna

CTSH siRNA (Mouse)

Cat. Num. AAA8215802

• Gene Names: Ctsh; AL022844
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CTSH; CTSH siRNA (Mouse); Pro-cathepsin H; Cathepsin B3; Cathepsin BA; CTSH sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CTSH siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 333

• Applicable Applications for CTSH sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CTSH gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CTSH sirna

siRNA to inhibit CTSH expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 166235890
• NCBI GeneID: 13036
• NCBI Accession #: NP_031827.2
• NCBI GenBank Nucleotide #: NM_007801.3
• UniProt Accession #: P49935; Q3UCD6
• Molecular Weight: 37,170 Da
• NCBI Official Full Name: pro-cathepsin H isoform 1 preproprotein
• NCBI Official Synonym Full Names: cathepsin H
• NCBI Official Symbol: Ctsh
• NCBI Official Synonym Symbols: AL022844
• NCBI Protein Information: pro-cathepsin H
• UniProt Protein Name: Pro-cathepsin H
• Protein Family: Pro-cathepsin
• UniProt Gene Name: Ctsh
• UniProt Entry Name: CATH_MOUSE

NCBI Description

This gene encodes a member of the peptidase C1 (papain) family of cysteine proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include the cathepsin H mini, heavy, and light chains. In rat and human, these three chains can associate to form the mature enzyme, which has both aminopeptidase and endopeptidase activities. Homozygous knockout mice for this gene exhibit impaired lung surfactant processing and reduced tumorigenesis in a pancreatic cancer model. Multiple pseudogenes of this gene have been identified in the genome. [provided by RefSeq, Aug 2015]

Uniprot Description

CTSH: Important for the overall degradation of proteins in lysosomes. Belongs to the peptidase C1 family.

Protein type: Protease; EC 3.4.22.16

Cellular Component: extracellular space; lysosome; acrosome; axoneme; outer dense fiber; cytosol

Molecular Function: peptidase activity; protein self-association; HLA-A specific activating MHC class I receptor activity; hydrolase activity; cysteine-type endopeptidase activity; serine-type endopeptidase activity; endopeptidase activity; apoptotic protease activator activity; protein complex binding; caspase activator activity; kininogen binding; aminopeptidase activity; cysteine-type peptidase activity

Biological Process: caspase activation; positive regulation of angiogenesis; response to retinoic acid; protein destabilization; apoptosis; membrane protein proteolysis; positive regulation of cell proliferation; zymogen activation; proteolysis; surfactant homeostasis; immune response-regulating signal transduction; positive regulation of cell migration; negative regulation of apoptosis; T cell mediated cytotoxicity