CTSA sirna

CTSA siRNA (Mouse)

Cat. Num. AAA8215161

• Gene Names: Ctsa; PPCA; Ppgb; AU019505
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CTSA; CTSA siRNA (Mouse); PPGB; Lysosomal protective protein; Carboxypeptidase C; Carboxypeptidase L; Cathepsin A; Protective protein cathepsin A; PPCA; Protective protein for beta-galactosidase; CTSA sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CTSA siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 474

• Applicable Applications for CTSA sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CTSA gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CTSA sirna

siRNA to inhibit CTSA expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 84042523
• NCBI GeneID: 19025
• NCBI Accession #: NP_001033581.1
• NCBI GenBank Nucleotide #: NM_001038492.2
• UniProt Accession #: P16675; Q8VEF6
• Molecular Weight: 53,844 Da
• NCBI Official Full Name: lysosomal protective protein isoform b preproprotein
• NCBI Official Synonym Full Names: cathepsin A
• NCBI Official Symbol: Ctsa
• NCBI Official Synonym Symbols: PPCA; Ppgb; AU019505
• NCBI Protein Information: lysosomal protective protein
• UniProt Protein Name: Lysosomal protective protein
• Protein Family: Lysosomal protective protein
• UniProt Gene Name: Ctsa
• UniProt Synonym Gene Names: Ppgb; PPCA
• UniProt Entry Name: PPGB_MOUSE

NCBI Description

This gene encodes a glycoprotein with deamidase, esterase and carboxypeptidase activities. The encoded protein associates with and provides a protective function to the lysosomal enzymes beta-galactosidase and neuraminidase. Deficiency of the related gene in humans results in galactosialidosis. The proprotein is processed into two shorter chains. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]

Uniprot Description

CTSA: Protective protein appears to be essential for both the activity of beta-galactosidase and neuraminidase, it associates with these enzymes and exerts a protective function necessary for their stability and activity. This protein is also a carboxypeptidase and can deamidate tachykinins. Defects in CTSA are the cause of galactosialidosis (GSL). A lysosomal storage disease associated with a combined deficiency of beta-galactosidase and neuraminidase, secondary to a defect in cathepsin A. All patients have clinical manifestations typical of a lysosomal disorder, such as coarse facies, cherry red spots, vertebral changes, foam cells in the bone marrow, and vacuolated lymphocytes. Three phenotypic subtypes are recognized. The early infantile form is associated with fetal hydrops, edema, ascites, visceromegaly, skeletal dysplasia, and early death. The late infantile type is characterized by hepatosplenomegaly, growth retardation, cardiac involvement, and a normal or mildly affected mental state. The juvenile/adult form is characterized by myoclonus, ataxia, angiokeratoma, mental retardation, neurologic deterioration, absence of visceromegaly, and long survival. Belongs to the peptidase S10 family.

Protein type: Endoplasmic reticulum; Protease; EC 3.4.16.5; Mitochondrial

Cellular Component: nucleoplasm; mitochondrion; intracellular membrane-bound organelle; membrane; lysosome

Molecular Function: peptidase activity; serine carboxypeptidase activity; protein binding; hydrolase activity; carboxypeptidase activity

Biological Process: proteolysis