CTNNB1 sirna

CTNNB1 siRNA (Mouse)

Cat. Num. AAA8240841

• Gene Names: Ctnnb1; Bfc; Mesc; Catnb
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CTNNB1; CTNNB1 siRNA (Mouse); CATNB; Catenin beta-1; Beta-catenin; CTNNB1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CTNNB1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 781

• Applicable Applications for CTNNB1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CTNNB1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CTNNB1 sirna

siRNA to inhibit CTNNB1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 260166642
• NCBI GeneID: 12387
• NCBI Accession #: NP_001159374.1
• NCBI GenBank Nucleotide #: NM_001165902.1
• UniProt Accession #: Q02248; Q922W1; Q9D335
• Molecular Weight: 85,471 Da
• NCBI Official Full Name: catenin beta-1
• NCBI Official Synonym Full Names: catenin (cadherin associated protein), beta 1
• NCBI Official Symbol: Ctnnb1
• NCBI Official Synonym Symbols: Bfc; Mesc; Catnb
• NCBI Protein Information: catenin beta-1
• UniProt Protein Name: Catenin beta-1
• Protein Family: Catenin
• UniProt Gene Name: Ctnnb1
• UniProt Synonym Gene Names: Catnb
• UniProt Entry Name: CTNB1_MOUSE

NCBI Description

This gene encodes not only an important cytoplasmic component of the classical cadherin adhesion complex that forms the adherens junction in epithelia and mediates cell-cell adhesion in many other tissues but also a key signaling molecule in the canonical Wnt signaling pathway that controls cell growth and differentiation during both normal development and tumorigenesis. The gene product contains a central armadillo-repeat containing domain through which it binds the cytoplasmic tail of classical cadherins; meanwhile, it also binds alpha-catenin, which further links the cadherin complex to the actin cytoskeleton either directly or indirectly. Beta-catenin is therefore necessary for the adhesive function of classical cadherins. Another key function of this protein is to mediate the canonical Wnt signaling pathway and regulate gene transcription. Without Wnt signal, cytoplasmic beta-catenin that is not associated with the cadherin complex is quickly phosphorylated at the N-terminal Ser/Thr residues by the so called degradation complex containing axin, adenomatous polyposis coli (APC), casein kinase I, and GSK3B, then ubiquitylated by beta-TrCP, and degraded by the proteasome. However, in the presence of Wnt signal, the degradation complex is disrupted and the stabilized cytoplasmic beta-catenin translocates into the nucleus, where it binds various transcription factors and, together with these factors, regulates the transcription of many downstream genes. Mutations of this gene have been linked with various types of tumors. Alternatively spliced variants have been found for this gene. [provided by RefSeq, Sep 2009]

Uniprot Description

CTNNB1: a regulator of cell adhesion and a key downstream effector in the Wnt signaling pathway. Implicated early embryonic development and tumorigenesis. Phosphorylated and destabilized by CK1 and GSK-3beta. Stabilized cytoplasmic beta-catenin is a hallmark of a variety of cancers. Stabilized beta-catenin translocates to the nucleus, where it acts as a transcriptional activator of T-cell factor (TCF)-regulated genes. Interacts with the PDZ domain of TAX1BP3, inhibiting its transcriptional activity. Two alternatively spliced human isoforms have been described.

Protein type: Nuclear receptor co-regulator; Cell adhesion; Oncoprotein; Transcription factor; Motility/polarity/chemotaxis; Actin-binding

Cellular Component: centrosome; basolateral plasma membrane; intercellular junction; fascia adherens; beta-catenin destruction complex; cytosol; transcription factor complex; cell-cell adherens junction; membrane; cell projection membrane; lamellipodium; cytoplasm; lateral plasma membrane; apical junction complex; protein complex; tight junction; cell; catenin complex; Z disc; cytoskeleton; adherens junction; microvillus membrane; apical part of cell; plasma membrane; cell junction; nucleus

Molecular Function: protein binding; signal transducer activity; DNA binding; cadherin binding; double-stranded DNA binding; transcription coactivator activity; chromatin binding; transcription factor binding; protein phosphatase binding; transcription factor activity; nuclear hormone receptor binding; alpha-catenin binding

Biological Process: skin development; cellular morphogenesis during differentiation; regulation of myelination; positive regulation of transcription, DNA-dependent; negative regulation of chondrocyte differentiation; cell maturation; positive regulation of fibroblast growth factor receptor signaling pathway; cell fate determination; morphogenesis of embryonic epithelium; chromatin-mediated maintenance of transcription; embryonic foregut morphogenesis; positive regulation of mesenchymal cell proliferation; regulation of cell fate specification; male genitalia development; synapse organization and biogenesis; kidney development; T cell differentiation; positive regulation of neuroblast proliferation; nervous system development; transcription, DNA-dependent; regulation of osteoblast differentiation; regulation of transcription from RNA polymerase II promoter; regulation of gene expression; regulation of epithelial cell differentiation; smooth muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; embryonic forelimb morphogenesis; limb development; neuron migration; cardiac muscle cell proliferation; dorsal/ventral axis specification; negative regulation of transcription from RNA polymerase II promoter; positive regulation of MAPKKK cascade; pancreas development; forebrain development; positive regulation of cell proliferation; vasculogenesis; proximal/distal pattern formation; negative regulation of mitotic cell cycle, embryonic; Wnt receptor signaling pathway; in utero embryonic development; neural plate development; stem cell maintenance; gastrulation with mouth forming second; positive regulation of telomere maintenance via telomerase; regulation of cell proliferation; odontogenesis of dentine-containing teeth; positive regulation of osteoblast differentiation; cell proliferation; negative regulation of cell differentiation; dorsal/ventral pattern formation; ureteric bud branching; embryonic heart tube development; lens morphogenesis in camera-type eye; lung development; anterior/posterior axis specification; heart development; T cell differentiation in the thymus; osteoclast differentiation; Wnt receptor signaling pathway through beta-catenin; regulation of apoptosis; hair cycle process; cell-cell adhesion; positive regulation of endothelial cell differentiation; organ development; ectoderm development; positive regulation of epithelial cell differentiation; cell adhesion; bone resorption; positive regulation of I-kappaB kinase/NF-kappaB cascade; patterning of blood vessels; genitalia morphogenesis; endoderm formation; negative regulation of transcription, DNA-dependent; cellular process; oocyte development; negative regulation of osteoclast differentiation; glial cell fate determination; endodermal cell fate commitment; cell-matrix adhesion; cell fate specification; embryonic hindlimb morphogenesis; synaptic transmission; regulation of osteoclast differentiation; central nervous system vasculogenesis; vasculature development; regulation of transcription, DNA-dependent; regulation of cell differentiation; hemopoiesis; cell differentiation; skeletal development; fallopian tube development; layer formation in the cerebral cortex; hair follicle morphogenesis; thymus development; embryonic axis specification; regulation of T cell proliferation; synaptic vesicle transport; positive regulation of telomerase activity; negative regulation of oligodendrocyte differentiation