CRTC2 sirna

CRTC2 siRNA (Mouse)

Cat. Num. AAA8240188

• Gene Names: Crtc2; Torc2; mTORC2; 4632407F12Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CRTC2; CRTC2 siRNA (Mouse); TORC2; CREB-regulated transcription coactivator 2; Transducer of regulated cAMP response element-binding protein 2; TORC-2; Transducer of CREB protein 2; CRTC2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CRTC2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 692

• Applicable Applications for CRTC2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CRTC2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CRTC2 sirna

siRNA to inhibit CRTC2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 58037361
• NCBI GeneID: 74343
• NCBI Accession #: NP_083157.1
• NCBI GenBank Nucleotide #: NM_028881.2
• UniProt Accession #: Q3U182; Q3TA52; Q8BH09
• Molecular Weight: 73,216 Da
• NCBI Official Full Name: CREB-regulated transcription coactivator 2
• NCBI Official Synonym Full Names: CREB regulated transcription coactivator 2
• NCBI Official Symbol: Crtc2
• NCBI Official Synonym Symbols: Torc2; mTORC2; 4632407F12Rik
• NCBI Protein Information: CREB-regulated transcription coactivator 2
• UniProt Protein Name: CREB-regulated transcription coactivator 2
• Protein Family: CREB-regulated transcription coactivator
• UniProt Gene Name: Crtc2
• UniProt Synonym Gene Names: Torc2; TORC-2; Transducer of CREB protein 2
• UniProt Entry Name: CRTC2_MOUSE

Uniprot Description

TORC2: the transducer of regulated CREB protein 2 (TORC2) is a cAMP responsive coactivator that, in concert with LKB1 and AMPK, controls glucose homeostasis in the liver. Under fasting conditions, cytoplasmic TORC2 is transported to the nucleus where it binds to CREB and stimulates gluconeogenisis. Re-feeding and the ensuing insulin response inhibits gluconeogenic gene expression by promoting the phosphorylation, cytoplasmic export and degradation of TORC2. TORC2 is phosphorylated by the kinase QIK which in turn is activated by phosphorylation at Ser358 by insulin-activated Akt2. Phosphorylated TORC2 is subsequently ubiquitinylated at Lys 628 and degraded by the 26S proteasome. TORC2 protein levels and activity are increased in diabetes owing to a block in TORC2 phosphorylation, implicating this pathway in the pathogenesis of diabetes. The CREB/TORC2 regulatory axis controls the normal pattern of germinal center (GC) B cell gene activation/repression that promotes B cell development and circumvents GC lymphomagenesis.

Protein type: Transcription, coactivator/corepressor

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; cAMP response element binding protein binding; chromatin binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; activation of CREB transcription factor; positive regulation of transcription from RNA polymerase II promoter; glucose homeostasis; protein homotetramerization; gluconeogenesis