COPB1 sirna

COPB1 siRNA (Mouse)

Cat. Num. AAA828431

• Gene Names: Copb1; 2610019B04Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: COPB1; COPB1 siRNA (Mouse); COPB; Coatomer subunit beta; Beta-coat protein; Beta-COP; COPB1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: COPB1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 953

• Applicable Applications for COPB1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse COPB1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for COPB1 sirna

siRNA to inhibit COPB1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 15426055
• NCBI GeneID: 70349
• NCBI Accession #: NP_203534.1
• NCBI GenBank Nucleotide #: NM_033370.3
• UniProt Accession #: Q9JIF7; Q3T9Y4; Q3TT72; Q3U8G9; Q3UE02
• Molecular Weight: 107,066 Da
• NCBI Official Full Name: coatomer subunit beta
• NCBI Official Synonym Full Names: coatomer protein complex, subunit beta 1
• NCBI Official Symbol: Copb1
• NCBI Official Synonym Symbols: 2610019B04Rik
• NCBI Protein Information: coatomer subunit beta
• UniProt Protein Name: Coatomer subunit beta
• UniProt Gene Name: Copb1
• UniProt Synonym Gene Names: Copb; Beta-COP
• UniProt Entry Name: COPB_MOUSE

Uniprot Description

COPB1: The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Plays a functional role in facilitating the transport of kappa- type opioid receptor mRNAs into axons and enhances translation of these proteins. Required for limiting lipid storage in lipid droplets. Involved in lipid homeostasis by regulating the presence of perilipin family members PLIN2 and PLIN3 at the lipid droplet surface and promoting the association of adipocyte surface triglyceride lipase (PNPLA2) with the lipid droplet to mediate lipolysis. Involved in the Golgi disassembly and reassembly processes during cell cycle. Involved in autophagy by playing a role in early endosome function. Plays a role in organellar compartmentalization of secretory compartments including endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC), Golgi, trans-Golgi network (TGN) and recycling endosomes, and in biosynthetic transport of CAV1. Promotes degradation of Nef cellular targets CD4 and MHC class I antigens by facilitating their trafficking to degradative compartments.

Protein type: Vesicle; Motility/polarity/chemotaxis

Cellular Component: Golgi apparatus; COPI-coated vesicle; membrane coat; intracellular membrane-bound organelle; membrane; cytoplasm; plasma membrane; Golgi-associated vesicle; COPI vesicle coat; cytoplasmic vesicle

Molecular Function: structural molecule activity

Biological Process: vesicle-mediated transport; intracellular protein transport; intra-Golgi vesicle-mediated transport; protein transport; transport