COL4A2 sirna

COL4A2 siRNA (Mouse)

Cat. Num. AAA8203279

• Gene Names: Col4a2; Col4a-2
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: COL4A2; COL4A2 siRNA (Mouse); Collagen alpha-2(IV) chain; COL4A2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: COL4A2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1707

• Applicable Applications for COL4A2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse COL4A2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for COL4A2 sirna

siRNA to inhibit COL4A2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 226437587
• NCBI GeneID: 12827
• NCBI Accession #: NP_034062.3
• NCBI GenBank Nucleotide #: NM_009932.4
• UniProt Accession #: P08122; Q3B7C2; Q3TPV9; Q61375; Q66JS5; Q6RCT6; Q6RCT7; Q6U9X1; Q8BPI9; Q8BPK3; Q91VI3
• Molecular Weight: 167,325 Da
• NCBI Official Full Name: collagen alpha-2(IV) chain preproprotein
• NCBI Official Synonym Full Names: collagen, type IV, alpha 2
• NCBI Official Symbol: Col4a2
• NCBI Official Synonym Symbols: Col4a-2
• NCBI Protein Information: collagen alpha-2(IV) chain
• UniProt Protein Name: Collagen alpha-2(IV) chain
• Protein Family: Collagen
• UniProt Gene Name: Col4a2
• UniProt Entry Name: CO4A2_MOUSE

NCBI Description

This gene encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include the collagen alpha-2(IV) chain and canstatin. The collagen chain is a subunit of heterotrimeric type IV collagen, which comprises a major structural component of basement membranes. Canstatin, which is cleaved from the C-terminus of the collagen chain, is a matrikine that has been shown to inhibit angiogenesis. Homozygous knockout mice for this gene exhibit impaired basement membrane integrity and embryonic lethality. This gene shares a bi-directional promoter with a related gene on chromosome 8. [provided by RefSeq, Aug 2015]

Uniprot Description

COL4A2: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Defects in COL4A2 are the cause of porencephaly type 2 (POREN2). POREN2 is a neurologic disorder characterized by a fluid-filled cysts or cavities within the cerebral hemispheres. Affected individuals typically have hemiplegia, seizures, and intellectual disability. Porencephaly type 2, or schizencephalic porencephaly, is usually symmetric and represents a primary defect in the development of the cerebral ventricles. Defects in COL4A2 are a cause of susceptibility to intracerebral hemorrhage (ICH). ICH is a pathological condition characterized by bleeding into one or both cerebral hemispheres including the basal ganglia and the cerebral cortex. It is often associated with hypertension and craniocerebral trauma. Intracerebral bleeding is a common cause of stroke. Belongs to the type IV collagen family.

Protein type: Secreted, signal peptide; Secreted; Extracellular matrix

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; collagen; intracellular membrane-bound organelle; collagen type IV; extracellular region; basement membrane

Molecular Function: extracellular matrix structural constituent

Biological Process: negative regulation of angiogenesis; transcription, DNA-dependent; angiogenesis