CHMP2B sirna

CHMP2B siRNA (Mouse)

Cat. Num. AAA8218030

• Gene Names: Chmp2b; 1190006E07Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CHMP2B; CHMP2B siRNA (Mouse); Charged multivesicular body protein 2b; Chromatin-modifying protein 2b; CHMP2b; CHMP2B sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CHMP2B siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 213

• Applicable Applications for CHMP2B sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CHMP2B gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CHMP2B sirna

siRNA to inhibit CHMP2B expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 30794432
• NCBI GeneID: 68942
• NCBI Accession #: NP_081155.1
• NCBI GenBank Nucleotide #: NM_026879.2
• UniProt Accession #: Q8BJF9; Q80UZ4; Q9CT65
• Molecular Weight: 23,935 Da
• NCBI Official Full Name: charged multivesicular body protein 2b
• NCBI Official Synonym Full Names: charged multivesicular body protein 2B
• NCBI Official Symbol: Chmp2b
• NCBI Official Synonym Symbols: 1190006E07Rik
• NCBI Protein Information: charged multivesicular body protein 2b
• UniProt Protein Name: Charged multivesicular body protein 2b
• Protein Family: Charged multivesicular body protein
• UniProt Gene Name: Chmp2b
• UniProt Synonym Gene Names: CHMP2b
• UniProt Entry Name: CHM2B_MOUSE

Uniprot Description

CHMP2B: Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Defects in CHMP2B are the cause of frontotemporal dementia, chromosome 3-linked (FTD3). FTD3 is characterized by an onset of dementia in the late 50's initially characterized by behavioral and personality changes including apathy, restlessness, disinhibition and hyperorality, progressing to stereotyped behaviors, non-fluent aphasia, mutism and dystonia, with a marked lack of insight. The brains of individuals with FTD3 have no distinctive neuropathological features. They show global cortical and central atrophy, but no beta-amyloid deposits. Defects in CHMP2B are the cause of amyotrophic lateral sclerosis type 17 (ALS17). An adult-onset progressive neurodegenerative disorder with predominantly lower motor neuron involvement, manifest as muscle weakness and wasting of the upper and lower limbs, bulbar signs, and respiratory insufficiency. Belongs to the SNF7 family.

Cellular Component: nucleoplasm; mitochondrion; membrane; lysosome; late endosome; cytoplasm; plasma membrane; intracellular; nucleus; cytosol; endosome

Molecular Function: protein domain specific binding

Biological Process: protein transport; cell separation during cytokinesis; endosome organization and biogenesis; transport; vacuolar transport; cognition; mitotic metaphase plate congression; nuclear organization and biogenesis