CBX2 sirna

CBX2 siRNA (Mouse)

Cat. Num. AAA8208114

• Gene Names: Cbx2; pc; M33; MOD2
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CBX2; CBX2 siRNA (Mouse); M33; Chromobox protein homolog 2; Modifier 3 protein; CBX2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CBX2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 519

• Applicable Applications for CBX2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CBX2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CBX2 sirna

siRNA to inhibit CBX2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 31542346
• NCBI GeneID: 12416
• NCBI Accession #: NP_031649.2
• NCBI GenBank Nucleotide #: NM_007623.2
• UniProt Accession #: P30658; O35731; Q8CIA0; A2ABG2
• Molecular Weight: 54,918 Da
• NCBI Official Full Name: chromobox protein homolog 2
• NCBI Official Synonym Full Names: chromobox 2
• NCBI Official Symbol: Cbx2
• NCBI Official Synonym Symbols: pc; M33; MOD2
• NCBI Protein Information: chromobox protein homolog 2
• UniProt Protein Name: Chromobox protein homolog 2
• Protein Family: Chromobox protein
• UniProt Gene Name: Cbx2
• UniProt Synonym Gene Names: M33
• UniProt Entry Name: CBX2_MOUSE

NCBI Description

This gene encodes a component of the polycomb multiprotein complex, which is required to maintain the transcriptionally repressive state of many genes throughout development via chromatin remodeling and modification of histones. Disruption of this gene in results in male-to-female gonadal sex reversal. [provided by RefSeq, Sep 2015]

Uniprot Description

CBX2: Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Involved in sexual development, acting as activator of NR5A1 expression. Defects in CBX2 are the cause of 46,XY sex reversal type 5 (SRXY5). It is a disorder of sex development. Affected individuals have a 46,XY karyotype but present as phenotypically normal females. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription regulation

Cellular Component: nucleoplasm; heterochromatin; euchromatin; PcG protein complex; nucleus

Molecular Function: protein binding; DNA binding; chromatin binding; methylated histone residue binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; chromatin modification; negative regulation of transcription from RNA polymerase II promoter; development of primary sexual characteristics