CALCRL sirna

CALCRL siRNA (Mouse)

Cat. Num. AAA8231041

• Gene Names: Calcrl; CRLR; AV071593
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CALCRL; CALCRL siRNA (Mouse); CRLR; Calcitonin gene-related peptide type 1 receptor; CGRP type 1 receptor; Calcitonin receptor-like receptor; CALCRL sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: CALCRL siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 463

• Applicable Applications for CALCRL sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CALCRL gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CALCRL sirna

siRNA to inhibit CALCRL expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 213385308
• NCBI GeneID: 54598
• NCBI Accession #: NP_061252.2
• NCBI GenBank Nucleotide #: NM_018782.2
• UniProt Accession #: Q9R1W5; Q9QXH8; Q9WUP2
• Molecular Weight: 53,265 Da
• NCBI Official Full Name: calcitonin gene-related peptide type 1 receptor
• NCBI Official Synonym Full Names: calcitonin receptor-like
• NCBI Official Symbol: Calcrl
• NCBI Official Synonym Symbols: CRLR; AV071593
• NCBI Protein Information: calcitonin gene-related peptide type 1 receptor
• UniProt Protein Name: Calcitonin gene-related peptide type 1 receptor
• Protein Family: Calcitonin gene-related peptide type 1 receptor
• UniProt Gene Name: Calcrl
• UniProt Synonym Gene Names: Crlr; CGRP type 1 receptor
• UniProt Entry Name: CALRL_MOUSE

Uniprot Description

CALCRL: Receptor for calcitonin-gene-related peptide (CGRP) together with RAMP1 and receptor for adrenomedullin together with RAMP3. Receptor for adrenomedullin together with RAMP2. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Belongs to the G-protein coupled receptor 2 family.

Protein type: Membrane protein, integral; GPCR, family 2; Cell cycle regulation; Receptor, GPCR; Membrane protein, multi-pass

Cellular Component: membrane; lysosome; endoplasmic reticulum; integral to membrane; plasma membrane; endosome

Molecular Function: G-protein coupled receptor activity; signal transducer activity; transmembrane receptor activity; calcitonin gene-related polypeptide receptor activity; adrenomedullin receptor activity; protein transporter activity; calcitonin receptor activity

Biological Process: heart development; positive regulation of smooth muscle cell proliferation; signal transduction; G-protein signaling, adenylate cyclase activating pathway; G-protein coupled receptor protein signaling pathway; protein transport; cell surface receptor linked signal transduction; negative regulation of inflammatory response; receptor internalization; negative regulation of smooth muscle contraction; calcium ion transport; positive regulation of cAMP biosynthetic process; positive regulation of cell proliferation; cAMP biosynthetic process; angiogenesis