BRCA2 sirna

BRCA2 siRNA (Mouse)

Cat. Num. AAA8231032

• Gene Names: Brca2; Fancd1; RAB163
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BRCA2; BRCA2 siRNA (Mouse); FANCD1; Breast cancer type 2 susceptibility protein homolog; Fanconi anemia group D1 protein homolog; BRCA2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: BRCA2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 3,329

• Applicable Applications for BRCA2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse BRCA2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BRCA2 sirna

siRNA to inhibit BRCA2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 124487409
• NCBI GeneID: 12190
• NCBI Accession #: NP_001074470.1
• NCBI GenBank Nucleotide #: NM_001081001.2
• UniProt Accession #: P97929; O35922; P97383; F8VPU5
• Molecular Weight: 370,664 Da
• NCBI Official Full Name: breast cancer type 2 susceptibility protein homolog
• NCBI Official Synonym Full Names: breast cancer 2
• NCBI Official Symbol: Brca2
• NCBI Official Synonym Symbols: Fancd1; RAB163
• NCBI Protein Information: breast cancer type 2 susceptibility protein homolog
• UniProt Protein Name: Breast cancer type 2 susceptibility protein homolog
• Protein Family: BRCA2 and CDKN1A-interacting protein
• UniProt Gene Name: Brca2
• UniProt Synonym Gene Names: Fancd1
• UniProt Entry Name: BRCA2_MOUSE

Uniprot Description

BRCA2: involved in double-strand break repair and/or homologous recombination. May participate in S phase checkpoint activation. Interacts with RAD51 and DSS1. Interacts with ubiquitinated FANCD2. Interacts with PALB2, enables the recombinational repair and checkpoint functions. Interacts with WDR16. Defects in BRCA2 are a cause of genetic susceptibility to breast cancer and may underlie susceptibility to uveal melanoma.

Protein type: DNA repair, damage; Nuclear receptor co-regulator; Tumor suppressor

Cellular Component: nucleoplasm; centrosome; protein complex; cytoskeleton; cytoplasm; BRCA2-MAGE-D1 complex; intracellular; nucleus; secretory granule

Molecular Function: protein binding; gamma-tubulin binding; H4 histone acetyltransferase activity; DNA binding; protease binding; H3 histone acetyltransferase activity; single-stranded DNA binding

Biological Process: positive regulation of transcription, DNA-dependent; cytokinesis; positive regulation of mitotic cell cycle; chromosome organization and biogenesis; homologous chromosome orientation during meiosis; DNA recombination; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; regulation of transcription, DNA-dependent; mammary gland development; double-strand break repair; response to gamma radiation; hemopoiesis; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; female gonad development; response to X-ray; chordate embryonic development; multicellular organism growth; cell aging; cell cycle; DNA repair; regulation of cytokinesis; inner cell mass cell proliferation; oocyte maturation; response to UV-C; negative regulation of mammary gland epithelial cell proliferation; double-strand break repair via homologous recombination; regulation of cell proliferation; cell proliferation; DNA damage response, signal transduction resulting in induction of apoptosis; male meiosis I; nucleotide-excision repair; spermatogenesis; replication fork protection; brain development; response to DNA damage stimulus; centrosome duplication