BMX sirna

BMX siRNA (Mouse)

Cat. Num. AAA8201680

• Gene Names: Bmx; Etk; Tyro8; Etk/Bmx
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BMX; BMX siRNA (Mouse); Cytoplasmic tyrosine-protein kinase BMX; Bone marrow tyrosine kinase gene in chromosome X protein homolog; BMX sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: BMX siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 655

• Applicable Applications for BMX sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse BMX gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BMX sirna

siRNA to inhibit BMX expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 226693402
• NCBI GeneID: 12169
• NCBI Accession #: NP_033889.2
• NCBI GenBank Nucleotide #: NM_009759.4
• UniProt Accession #: P97504
• Molecular Weight: 75,001 Da
• NCBI Official Full Name: cytoplasmic tyrosine-protein kinase BMX
• NCBI Official Synonym Full Names: BMX non-receptor tyrosine kinase
• NCBI Official Symbol: Bmx
• NCBI Official Synonym Symbols: Etk; Tyro8; Etk/Bmx
• NCBI Protein Information: cytoplasmic tyrosine-protein kinase BMX
• UniProt Protein Name: Cytoplasmic tyrosine-protein kinase BMX
• Protein Family: Cytoplasmic tyrosine-protein kinase
• UniProt Gene Name: Bmx
• UniProt Entry Name: BMX_MOUSE

Uniprot Description

Etk: a tyrosine kinase of the Tec family. Activity is required for IL6-induced differentiation. May play a role in the growth and differentiation of hematopoietic cells. May be involved in signal transduction in endocardial and arterial endothelial cells.

Protein type: Protein kinase, tyrosine (non-receptor); Kinase, protein; EC 2.7.10.2; Protein kinase, TK; TK group; Tec family

Cellular Component: extrinsic to internal side of plasma membrane; cytoplasm

Molecular Function: transferase activity; metal ion binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; kinase activity; ATP binding; protein kinase activity

Biological Process: adaptive immune response; peptidyl-tyrosine phosphorylation; apoptosis; protein amino acid autophosphorylation; innate immune response; cell adhesion; cell differentiation; signal transduction; protein amino acid phosphorylation; phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of cell proliferation