BIRC2 sirna

BIRC2 siRNA (Mouse)

Cat. Num. AAA8240710

• Gene Names: Birc2; Api1; Api2; IAP1; IAP2; MIHB; MIHC; Birc3; HIAP1; HIAP2; MIAP1; MIAP2; RNF48; cIAP1; cIAP2; mcIAP1; AW146227; C330006D17Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BIRC2; BIRC2 siRNA (Mouse); BIRC3; IAP2; Baculoviral IAP repeat-containing protein 2; Inhibitor of apoptosis protein 2; IAP-2; mIAP-2; mIAP2; BIRC2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: BIRC2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 612

• Applicable Applications for BIRC2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse BIRC2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BIRC2 sirna

siRNA to inhibit BIRC2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 160333366
• NCBI GeneID: 11797
• NCBI Accession #: NP_031491.2
• NCBI GenBank Nucleotide #: NM_007465.3
• UniProt Accession #: Q62210; O08864; A6H6S7
• Molecular Weight: 69,676 Da
• NCBI Official Full Name: baculoviral IAP repeat-containing protein 2
• NCBI Official Synonym Full Names: baculoviral IAP repeat-containing 2
• NCBI Official Symbol: Birc2
• NCBI Official Synonym Symbols: Api1; Api2; IAP1; IAP2; MIHB; MIHC; Birc3; HIAP1; HIAP2; MIAP1; MIAP2; RNF48; cIAP1; cIAP2; mcIAP1; AW146227; C330006D17Rik
• NCBI Protein Information: baculoviral IAP repeat-containing protein 2
• UniProt Protein Name: Baculoviral IAP repeat-containing protein 2
• Protein Family: Baculoviral IAP repeat-containing protein
• UniProt Gene Name: Birc2
• UniProt Synonym Gene Names: Birc3; Iap2; IAP-2; mIAP-2; mIAP2
• UniProt Entry Name: BIRC2_MOUSE

Uniprot Description

BIRC2: Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, mitogenic kinase signaling, and cell proliferation, as well as cell invasion and metastasis. Acts as an E3 ubiquitin- protein ligase regulating NF-kappa-B signaling and regulates both canonical and non-canonical NF-kappa-B signaling by acting in opposite directions: acts as a positive regulator of the canonical pathway and suppresses constitutive activation of non-canonical NF-kappa-B signaling. The target proteins for its E3 ubiquitin- protein ligase activity include: RIPK1, RIPK2, RIPK3, RIPK4, CASP3, CASP7, CASP8, TRAF2, DIABLO/SMAC, MAP3K14/NIK, MAP3K5/ASK1, IKBKG/NEMO and MXD1/MAD1. Can also function as an E3 ubiquitin- protein ligase of the NEDD8 conjugation pathway, targeting effector caspases for neddylation and inactivation. Acts as an important regulator of innate immune signaling via regulation of Toll-like receptors (TLRs), Nodlike receptors (NLRs) and RIG-I like receptors (RLRs), collectively referred to as pattern recognition receptors (PRRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8. Can stimulate the transcriptional activity of E2F1. Plays a role in the modulation of the cell cycle. Interacts with DIABLO/SMAC and with PRSS25; these interactions inhibit apoptotic suppressor activity. Interacts with CASP9. Interacts (via BIR domains) with TRAF2. Interacts with E2F1, RIPK1, RIPK2, RIPK3, RIPK4, BIRC5/survivin and USP19. Present in many fetal and adult tissues. Mainly expressed in adult skeletal muscle, thymus, testis, ovary, and pancreas, low or absent in brain and peripheral blood leukocytes. The CARD domain inhibits the activation of E3 ubiquitin ligase activity by preventing RING domain dimerization and E2 ubiquitin donor binding and activation. The CARD domain- mediated autoinhibition of the E3 ubiquitin-protein ligase activity suppresses cell proliferation and migration. USP19 regulates the stability of BIRC2/c-IAP1 by preventing its ubiquitination. Belongs to the IAP family.

Protein type: Ligase; EC 6.3.2.-; Ubiquitin conjugating system; Ubiquitin ligase

Cellular Component: XY body; internal side of plasma membrane; protein complex; cytoplasm; nucleus; lipid raft

Molecular Function: protein binding; zinc ion binding; coenzyme F420-2 alpha-glutamyl ligase activity; metal ion binding; UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity; transcription coactivator activity; caspase inhibitor activity; ribosomal S6-glutamic acid ligase activity; ubiquitin-protein ligase activity; protein N-terminus binding; coenzyme F420-0 gamma-glutamyl ligase activity; ligase activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; regulation of toll-like receptor signaling pathway; protein polyubiquitination; transcription, DNA-dependent; protein heterooligomerization; apoptosis; regulation of cell cycle; protein ubiquitination; cytokinesis; regulation of apoptosis; regulation of transcription, DNA-dependent; response to hypoxia; negative regulation of apoptosis; placenta development