BIN1 sirna

BIN1 siRNA (Mouse)

Cat. Num. AAA8227033

• Gene Names: Bin1; ALP-1; Amphl; SH3P9; BRAMP-2
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BIN1; BIN1 siRNA (Mouse); AMPHL; SH3P9; Myc box-dependent-interacting protein 1; Amphiphysin II; Amphiphysin-like protein; Bridging integrator 1; SH3 domain-containing protein 9; BIN1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: BIN1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 434

• Applicable Applications for BIN1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse BIN1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BIN1 sirna

siRNA to inhibit BIN1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6753050
• NCBI GeneID: 30948
• NCBI Accession #: NP_033798.1
• NCBI GenBank Nucleotide #: NM_009668.2
• UniProt Accession #: O08539; Q62434
• Molecular Weight: 48,014 Da
• NCBI Official Full Name: myc box-dependent-interacting protein 1 isoform 1
• NCBI Official Synonym Full Names: bridging integrator 1
• NCBI Official Symbol: Bin1
• NCBI Official Synonym Symbols: ALP-1; Amphl; SH3P9; BRAMP-2
• NCBI Protein Information: myc box-dependent-interacting protein 1
• UniProt Protein Name: Myc box-dependent-interacting protein 1
• Protein Family: Myc box-dependent-interacting protein
• UniProt Gene Name: Bin1
• UniProt Synonym Gene Names: Amphl; Sh3p9
• UniProt Entry Name: BIN1_MOUSE

Uniprot Description

BIN1: May be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation. Defects in BIN1 are the cause of centronuclear myopathy type 2 (CNM2). A congenital muscle disorder characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers. 11 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor; Adaptor/scaffold; Vesicle; Cytoskeletal

Cellular Component: I band; synaptic vesicle; membrane; axon; T-tubule; cytoplasm; nerve terminal; Z disc; nucleus

Molecular Function: identical protein binding; GTPase binding; protein binding; protein heterodimerization activity; protein complex binding; tau protein binding

Biological Process: muscle cell differentiation; positive regulation of apoptosis; regulation of neuron differentiation; multicellular organismal development; regulation of endocytosis; endocytosis; positive regulation of endocytosis; cell differentiation; positive regulation of astrocyte differentiation; positive regulation of GTPase activity