BCLAF1 sirna

BCLAF1 siRNA (Mouse)

Cat. Num. AAA829259

• Gene Names: Bclaf1; Btf; AI450190; AW556225; mKIAA0164; 2610102K23Rik; 2700025J07Rik; 2810454G14Rik; 5730534O06Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: BCLAF1; BCLAF1 siRNA (Mouse); BTF; KIAA0164; Bcl-2-associated transcription factor 1; Btf; BCLAF1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: BCLAF1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 919

• Applicable Applications for BCLAF1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse BCLAF1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for BCLAF1 sirna

siRNA to inhibit BCLAF1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 70906447
• NCBI GeneID: 72567
• NCBI Accession #: NP_001020563.1
• NCBI GenBank Nucleotide #: NM_001025392.1
• UniProt Accession #: Q8K019; Q8BNZ0; Q8C2E9; Q9CSW5
• Molecular Weight: 86,036 Da
• NCBI Official Full Name: bcl-2-associated transcription factor 1 isoform 1
• NCBI Official Synonym Full Names: BCL2-associated transcription factor 1
• NCBI Official Symbol: Bclaf1
• NCBI Official Synonym Symbols: Btf; AI450190; AW556225; mKIAA0164; 2610102K23Rik; 2700025J07Rik; 2810454G14Rik; 5730534O06Rik
• NCBI Protein Information: bcl-2-associated transcription factor 1
• UniProt Protein Name: Bcl-2-associated transcription factor 1
• Protein Family: Bcl-2-associated transcription factor
• UniProt Gene Name: Bclaf1
• UniProt Synonym Gene Names: Btf; Kiaa0164; Btf
• UniProt Entry Name: BCLF1_MOUSE

Uniprot Description

BCLAF1: a ubiquitous death-promoting transcriptional repressor. Interacts with Bcl-2 related proteins, lamin A, emerin, the adenovirus E1B 19 kDa protein and DNA. An emerin mutant S54F, which binds normally to barrier-to-autointegration factor (BAF), lamin A and GCL, selectively disrupts its binding to BCLAF1. BCLAF1 is found in dot-like structures throughout the nuclear interior in HeLa cells. Proapoptotic stimuli induce its redistribution to a distinct zone near the nuclear envelope. Four alternatively spliced human isoforms have been described.

Protein type: RNA-binding; Transcription factor

Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; DNA binding

Biological Process: regulation of transcription in response to stress; transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of apoptosis; apoptosis; negative regulation of transcription, DNA-dependent