B3GAT3 sirna

B3GAT3 siRNA (Mouse)

Cat. Num. AAA8205520

• Gene Names: B3gat3; 2810405M13Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: B3GAT3; B3GAT3 siRNA (Mouse); Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3; Beta-1.3-glucuronyltransferase 3; Glucuronosyltransferase I; GlcAT-I; UDP-GlcUA:Gal beta-1.3-Gal-R glucuronyltransferase; GlcUAT-I; B3GAT3 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: B3GAT3 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 335

• Applicable Applications for B3GAT3 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse B3GAT3 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for B3GAT3 sirna

siRNA to inhibit B3GAT3 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 13195672
• NCBI GeneID: 72727
• NCBI Accession #: NP_077218.1
• NCBI GenBank Nucleotide #: NM_024256.2
• UniProt Accession #: P58158
• Molecular Weight: 37,067 Da
• NCBI Official Full Name: galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3
• NCBI Official Synonym Full Names: beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I)
• NCBI Official Symbol: B3gat3
• NCBI Official Synonym Symbols: 2810405M13Rik
• NCBI Protein Information: galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3
• UniProt Protein Name: Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3
• Protein Family: Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase
• UniProt Gene Name: B3gat3
• UniProt Synonym Gene Names: GlcAT-I; GlcUAT-I
• UniProt Entry Name: B3GA3_MOUSE

Uniprot Description

B3GAT3: Glycosaminoglycans biosynthesis. Involved in forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate. Transfers a glucuronic acid moiety from the uridine diphosphate-glucuronic acid (UDP-GlcUA) to the common linkage region trisaccharide Gal-beta-1,3-Gal-beta-1,4-Xyl covalently bound to a Ser residue at the glycosaminylglycan attachment site of proteoglycans. Can also play a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins. Shows strict specificity for Gal-beta-1,3-Gal-beta-1,4-Xyl, exhibiting negligible incorporation into other galactoside substrates including Galbeta1-3Gal beta1-O-benzyl, Galbeta1-4GlcNAc and Galbeta1-4Glc. Defects in B3GAT3 are the cause of multiple joint dislocations short stature craniofacial dysmorphism and congenital heart defects (JDSSDHD). An autosomal recessive disease characterized by dysmorphic facies, bilateral dislocations of the elbows, hips, and knees, clubfeet, and short stature, as well as cardiovascular defects. Belongs to the glycosyltransferase 43 family.

Protein type: Membrane protein, integral; Glycan Metabolism - chondroitin sulfate biosynthesis; Transferase; Glycan Metabolism - heparan sulfate biosynthesis; EC 2.4.1.135

Cellular Component: Golgi apparatus; cis-Golgi network; membrane; integral to membrane

Molecular Function: transferase activity; glucuronosyltransferase activity; metal ion binding; galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity

Biological Process: positive regulation of catalytic activity; glycosaminoglycan biosynthetic process; heparan sulfate proteoglycan biosynthetic process; chondroitin sulfate proteoglycan biosynthetic process; dermatan sulfate proteoglycan biosynthetic process