ATP5J sirna

ATP5J siRNA (Mouse)

Cat. Num. AAA8238349

• Gene Names: Atp5j; CF6
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ATP5J; ATP5J siRNA (Mouse); ATP synthase-coupling factor 6 mitochondrial; ATPase subunit F6; ATP5J sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ATP5J siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 108

• Applicable Applications for ATP5J sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ATP5J gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ATP5J sirna

siRNA to inhibit ATP5J expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 695271688
• NCBI GeneID: 11957
• NCBI Accession #: NP_001289142.1
• NCBI GenBank Nucleotide #: NM_001302213.1
• UniProt Accession #: P97450
• Molecular Weight: 12,496 Da
• NCBI Official Full Name: ATP synthase-coupling factor 6, mitochondrial
• NCBI Official Synonym Full Names: ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F
• NCBI Official Symbol: Atp5j
• NCBI Official Synonym Symbols: CF6
• NCBI Protein Information: ATP synthase-coupling factor 6, mitochondrial
• UniProt Protein Name: ATP synthase-coupling factor 6, mitochondrial
• Protein Family: ATP synthase-coupling factor
• UniProt Gene Name: Atp5j
• UniProt Synonym Gene Names: ATPase subunit F6
• UniProt Entry Name: ATP5J_MOUSE

NCBI Description

The protein encoded by this gene is a component of mitochondrial adenosine triphosphate synthase, which catalyzes the conversion of ATP from ADP. Mitochondrial adenosine triphosphate synthase consists of extrinsic and intrinsic membrane domains that are joined by a stalk. The protein encoded by this gene is a subunit of the stalk domain. A bi-directional promoter that drives expression of this gene has been has been identified. Pseudogenes of this gene are found on chromosomes 14 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

Uniprot Description

ATP5J: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. Also involved in the restoration of oligomycin-sensitive ATPase activity to depleted F1-F0 complexes. Belongs to the eukaryotic ATPase subunit F6 family.

Protein type: EC 3.6.3.14; Energy Metabolism - oxidative phosphorylation; Mitochondrial

Cellular Component: membrane; mitochondrion; proton-transporting ATP synthase complex, coupling factor F(o); mitochondrial inner membrane; mitochondrial proton-transporting ATP synthase complex

Molecular Function: ATPase activity; hydrogen ion transmembrane transporter activity

Biological Process: ATP metabolic process; proton transport; ATP synthesis coupled proton transport; transport; ion transport