ARX sirna

ARX siRNA (Mouse)

Cat. Num. AAA8214968

• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ARX; ARX siRNA (Mouse); Homeobox protein ARX; Aristaless-related homeobox; ARX sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ARX siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 564

• Applicable Applications for ARX sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ARX gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ARX sirna

siRNA to inhibit ARX expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 806776662
• NCBI GeneID: 11878
• NCBI Accession #: NP_001292869.1
• NCBI GenBank Nucleotide #: NM_001305940.1
• UniProt Accession #: O35085; Q9QYT4; A2A4G9
• Molecular Weight: 58,490 Da
• NCBI Official Full Name: homeobox protein ARX
• NCBI Official Synonym Full Names: aristaless related homeobox
• NCBI Official Symbol: Arx
• NCBI Protein Information: homeobox protein ARX
• UniProt Protein Name: Homeobox protein ARX
• Protein Family: Aristaless-related homeobox protein
• UniProt Gene Name: Arx
• UniProt Entry Name: ARX_MOUSE

NCBI Description

This gene encodes a transcription factor that plays an important role in the development of forebrain. Male mice lacking this gene have smaller brains, olfactory bulbs and testes, and die within half a day after birth. Mice lacking this gene specifically in ganglionic eminence-derived neurons, including cortical interneurons, develop seizures. Mutations in this gene have been demonstrated to cause mouse phenotypes resembling human X-linked lissencephaly and mental retardation with epilepsy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]

Uniprot Description

ARX: Transcription factor required for normal brain development. May be important for maintenance of specific neuronal subtypes in the cerebral cortex and axonal guidance in the floor plate. Defects in ARX are the cause of lissencephaly X-linked type 2 (LISX2); also known as lissencephaly X-linked with ambiguous genitalia (XLAG). LISX2 is a classic type lissencephaly associated with abnormal genitalia. LISX2 patients have severe congenital or postnatal microcephaly, lissencephaly, agenesis of the corpus callosum, neonatal-onset intractable epilepsy, poor temperature regulation, chronic diarrhea, and ambiguous or underdeveloped genitalia. Defects in ARX are the cause of epileptic encephalopathy early infantile type 1 (EIEE1); also known as myoclonic epilepsy X-linked with intellectual disability and spasticity, X-linked West syndrome or X-linked infantile spasm syndrome (ISSX). EIEE1 is a severe form of epilepsy characterized by frequent tonic seizures or spasms beginning in infancy with a specific EEG finding of suppression-burst patterns, characterized by high-voltage bursts alternating with almost flat suppression phases. Patients may progress to West syndrome, which is characterized by tonic spasms with clustering, arrest of psychomotor development, and hypsarrhythmia on EEG. Defects in ARX are a cause of Partington syndrome (PRTS); also known as X-linked syndromic mental retardation 1 (MRXS1). PRTS is characterized by mental retardation, episodic dystonic hand movements, and dysarthria. Defects in ARX are the cause of mental retardation X- linked ARX-related (MRXARX). Mental retardation is a mental disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Defects in ARX are the cause of agenesis of the corpus callosum with abnormal genitalia (ACCAG). A X-linked syndrome with variable expression in females. It is characterized by agenesis of corpus callosum, mental retardation and seizures. Manifestations in surviving males include severe acquired micrencephaly, mental retardation, limb contractures, scoliosis, tapered fingers with hyperconvex nails, a characteristic face with large eyes, prominent supraorbital ridges, synophrys, optic atrophy, broad alveolar ridges, and seizures. Urologic anomalies include renal dysplasia, cryptorchidism, and hypospadias. Belongs to the paired homeobox family. Bicoid subfamily.

Protein type: DNA-binding; Cancer Testis Antigen (CTA)

Cellular Component: nucleus

Molecular Function: DNA binding; sequence-specific DNA binding; chromatin binding

Biological Process: transcription from RNA polymerase II promoter; nervous system development; axon guidance; embryonic olfactory bulb interneuron precursor migration; cerebral cortex GABAergic interneuron migration; transcription, DNA-dependent; cerebral cortex tangential migration; cell proliferation in forebrain; olfactory bulb development; multicellular organismal development; neuron migration; globus pallidus development; negative regulation of transcription from RNA polymerase II promoter; positive regulation of organ growth; regulation of cell proliferation; regulation of transcription, DNA-dependent; forebrain development; cell differentiation; lipid digestion