AP3B1 sirna

AP3B1 siRNA (Mouse)

Cat. Num. AAA8206833

• Gene Names: Ap3b1; pe; AP-3; Hps2; rim2; pearl; C78395; beta3A; AU015684
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AP3B1; AP3B1 siRNA (Mouse); AP-3 complex subunit beta-1; Adapter-related protein complex 3 subunit beta-1; Adaptor protein complex AP-3 subunit beta-1; Beta-3A-adaptin; Clathrin assembly protein complex 3 beta-1 large chain; AP3B1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: AP3B1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,105

• Applicable Applications for AP3B1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse AP3B1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AP3B1 sirna

siRNA to inhibit AP3B1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 163310776
• NCBI GeneID: 11774
• NCBI Accession #: NP_033810.2
• NCBI GenBank Nucleotide #: NM_009680.3
• UniProt Accession #: Q9Z1T1; Q91YR4; E9QQ08
• Molecular Weight: 122,740 Da
• NCBI Official Full Name: AP-3 complex subunit beta-1
• NCBI Official Synonym Full Names: adaptor-related protein complex 3, beta 1 subunit
• NCBI Official Symbol: Ap3b1
• NCBI Official Synonym Symbols: pe; AP-3; Hps2; rim2; pearl; C78395; beta3A; AU015684
• NCBI Protein Information: AP-3 complex subunit beta-1
• UniProt Protein Name: AP-3 complex subunit beta-1
• Protein Family: AP-3 complex
• UniProt Gene Name: Ap3b1
• UniProt Entry Name: AP3B1_MOUSE

Uniprot Description

AP3B1: a subunit of non-clathrin- and clathrin-associated adaptor protein complex 3 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related organelles. Mutations in AP3B1 cause defects of various cytoplasmic organelles including melanosomes, platelet dense granules and lysosomes, and are the cause of Hermansky-Pudlak syndrome 2. Two alternatively spliced isoforms have been described.

Protein type: Adaptor/scaffold; Vesicle

Cellular Component: Golgi apparatus; membrane coat; membrane; cell; lysosomal membrane; trans-Golgi network; intracellular; cytoplasmic vesicle

Molecular Function: GTP-dependent protein binding; protein phosphatase binding

Biological Process: vesicle-mediated transport; intracellular protein transport; antigen processing and presentation; protein targeting to lysosome; protein transport; transport; melanosome organization and biogenesis; anterograde synaptic vesicle transport; antigen processing and presentation, exogenous lipid antigen via MHC class Ib; positive regulation of NK T cell differentiation; zinc ion transport; blood coagulation; anterograde axon cargo transport