AP2M1 sirna

AP2M1 siRNA (Mouse)

Cat. Num. AAA8239101

• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AP2M1; AP2M1 siRNA (Mouse); CLAPM1; AP-2 complex subunit mu; AP-2 mu chain; Adapter-related protein complex 2 subunit mu; Adaptor protein complex AP-2 subunit mu; Clathrin assembly protein complex 2 mu medium chain; Clathrin coat assembly protein AP50; Clathrin coat-associated protein AP50; Mu2-adaptin; Plasma membrane adaptor AP-2 50 kDa protein; AP2M1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: AP2M1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 435

• Applicable Applications for AP2M1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse AP2M1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AP2M1 sirna

siRNA to inhibit AP2M1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6753074
• NCBI GeneID: 11773
• NCBI Accession #: NP_033809.1
• NCBI GenBank Nucleotide #: NM_009679.3
• UniProt Accession #: P84091; P20172; P53679
• Molecular Weight: 49,655 Da
• NCBI Official Full Name: AP-2 complex subunit mu isoform 1
• NCBI Official Synonym Full Names: adaptor-related protein complex 2, mu 1 subunit
• NCBI Official Symbol: Ap2m1
• NCBI Protein Information: AP-2 complex subunit mu
• UniProt Protein Name: AP-2 complex subunit mu
• UniProt Gene Name: Ap2m1
• UniProt Synonym Gene Names: Clapm1
• UniProt Entry Name: AP2M1_MOUSE

NCBI Description

This gene encodes the mu subunit of the clathrin adaptor protein complex AP-2. It mediates sorting of cargo proteins harboring Y-X-X-Phi motifs into clathrin-coated vesicles. Alternate splicing of this gene results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 2, 8 and 19. [provided by RefSeq, Dec 2014]

Uniprot Description

AP2M1: a protein of the adaptor complexes medium subunit family. A subunit of the heterotetrameric coat assembly protein complex 2 (AP2) which links clathrin to receptors in coated vesicles. Interacts with the cytoplasmic tails of membrane proteins and polyphosphoinositide-containing lipids. Is not required for clathrin-coated vesicle formation at the plasma membrane, but that it is one of several endocytic adaptors required for the uptake of certain cargo proteins. Is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. AP-2 is a heterotetramer composed of two large chains (alpha and beta), a medium chain (AP50) and a small chain (AP17).

Protein type: Vesicle; Adaptor/scaffold

Cellular Component: clathrin adaptor complex; mitochondrion; membrane; plasma membrane; terminal button; coated pit; secretory granule

Molecular Function: low-density lipoprotein receptor binding; signal sequence binding; lipid binding

Biological Process: vesicle-mediated transport; intracellular protein transport; protein transport; transport; endocytosis