AP1M1 sirna

AP1M1 siRNA (Mouse)

Cat. Num. AAA8235387

• Gene Names: Ap1m1; AP47; mu1A; Cltnm; [m]1A; Adtm1A; AA408894
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AP1M1; AP1M1 siRNA (Mouse); CLTNM; AP-1 complex subunit mu-1; AP-mu chain family member mu1A; Adapter-related protein complex 1 subunit mu-1; Adaptor protein complex AP-1 subunit mu-1; Clathrin assembly protein complex 1 mu-1 medium chain 1; Clathrin coat assembly protein AP47; Clathrin coat-associated protein AP47; Golgi adaptor HA1/AP1 adaptin mu-1 subunit; Mu-adaptin 1; Mu1A-adaptin; AP1M1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: AP1M1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 423

• Applicable Applications for AP1M1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse AP1M1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AP1M1 sirna

siRNA to inhibit AP1M1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6671557
• NCBI GeneID: 11767
• NCBI Accession #: NP_031482.1
• NCBI GenBank Nucleotide #: NM_007456.4
• UniProt Accession #: P35585
• Molecular Weight: 48,543 Da
• NCBI Official Full Name: AP-1 complex subunit mu-1
• NCBI Official Synonym Full Names: adaptor-related protein complex AP-1, mu subunit 1
• NCBI Official Symbol: Ap1m1
• NCBI Official Synonym Symbols: AP47; mu1A; Cltnm; [m]1A; Adtm1A; AA408894
• NCBI Protein Information: AP-1 complex subunit mu-1
• UniProt Protein Name: AP-1 complex subunit mu-1
• Protein Family: AP-1 complex
• UniProt Gene Name: Ap1m1
• UniProt Synonym Gene Names: Cltnm
• UniProt Entry Name: AP1M1_MOUSE

Uniprot Description

AP1M1: Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. Belongs to the adaptor complexes medium subunit family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle

Cellular Component: Golgi apparatus; clathrin adaptor complex; membrane; cell; trans-Golgi network; intracellular; cytoplasmic vesicle

Molecular Function: protein binding

Biological Process: vesicle-mediated transport; intracellular protein transport; protein transport; melanosome organization and biogenesis; transport