ALX4 sirna

ALX4 siRNA (Mouse)

Cat. Num. AAA8222868

• Gene Names: Alx4; lst
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ALX4; ALX4 siRNA (Mouse); Homeobox protein aristaless-like 4; ALX-4; ALX4 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ALX4 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 399

• Applicable Applications for ALX4 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ALX4 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ALX4 sirna

siRNA to inhibit ALX4 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6671541
• NCBI GeneID: 11695
• NCBI Accession #: NP_031468.1
• NCBI GenBank Nucleotide #: NM_007442.3
• UniProt Accession #: O35137
• Molecular Weight: 42,763 Da
• NCBI Official Full Name: homeobox protein aristaless-like 4
• NCBI Official Synonym Full Names: aristaless-like homeobox 4
• NCBI Official Symbol: Alx4
• NCBI Official Synonym Symbols: lst
• NCBI Protein Information: homeobox protein aristaless-like 4
• UniProt Protein Name: Homeobox protein aristaless-like 4
• Protein Family: Homeobox protein
• UniProt Gene Name: Alx4
• UniProt Entry Name: ALX4_MOUSE

Uniprot Description

ALX4: Transcription factor involved in skull and limb development. Plays an essential role in craniofacial development, skin and hair follicle development. Defects in ALX4 are the cause of parietal foramina 2 (PFM2); also known as foramina parietalia permagna (FPP). PFM2 is an autosomal dominant disease characterized by oval defects of the parietal bones caused by deficient ossification around the parietal notch, which is normally obliterated during the fifth fetal month. PFM2 is also a clinical feature of Potocki- Shaffer syndrome. Defects in ALX4 are the cause of frontonasal dysplasia type 2 (FND2). The term frontonasal dysplasia describes an array of abnormalities affecting the eyes, forehead and nose and linked to midfacial dysraphia. The clinical picture is highly variable. Major findings include true ocular hypertelorism; broadening of the nasal root; median facial cleft affecting the nose and/or upper lip and palate; unilateral or bilateral clefting of the alae nasi; lack of formation of the nasal tip; anterior cranium bifidum occultum; a V-shaped or widow's peak frontal hairline. Defects in ALX4 are a cause of Potocki-Shaffer syndrome (POSHS). A contiguous gene syndrome caused by deletion of the 11p11.2 region. Belongs to the paired homeobox family.

Protein type: DNA-binding

Cellular Component: transcription factor complex; nucleus

Molecular Function: protein binding; DNA binding; sequence-specific DNA binding; protein heterodimerization activity; transcription factor activity

Biological Process: embryonic forelimb morphogenesis; transcription from RNA polymerase II promoter; muscle development; transcription, DNA-dependent; multicellular organismal development; palate development; embryonic hindlimb morphogenesis; pattern specification process; limb morphogenesis; post-embryonic development; embryonic skeletal morphogenesis; anterior/posterior pattern formation; regulation of transcription from RNA polymerase II promoter; regulation of apoptosis; gut development; hair follicle development; regulation of transcription, DNA-dependent; skeletal morphogenesis; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis