AGTR1A sirna

AGTR1A siRNA (Mouse)

Cat. Num. AAA8217057

• Gene Names: Agtr1a; AT1; AG2S; AT1a; AT2R1; Agtr1; AT2R1A; Agtr-1a; AI551199; Angtr-1a; 1810074K20Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AGTR1A; AGTR1A siRNA (Mouse); AGTR1; Type-1A angiotensin II receptor; Angiotensin II type-1A receptor; AT1A; AGTR1A sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: AGTR1A siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 359

• Applicable Applications for AGTR1A sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse AGTR1A gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AGTR1A sirna

siRNA to inhibit AGTR1A expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 28893437
• NCBI GeneID: 11607
• NCBI Accession #: NP_796296.1
• NCBI GenBank Nucleotide #: NM_177322.3
• UniProt Accession #: P29754
• Molecular Weight: 40,856 Da
• NCBI Official Full Name: type-1A angiotensin II receptor
• NCBI Official Synonym Full Names: angiotensin II receptor, type 1a
• NCBI Official Symbol: Agtr1a
• NCBI Official Synonym Symbols: AT1; AG2S; AT1a; AT2R1; Agtr1; AT2R1A; Agtr-1a; AI551199; Angtr-1a; 1810074K20Rik
• NCBI Protein Information: type-1A angiotensin II receptor
• UniProt Protein Name: Type-1A angiotensin II receptor
• Protein Family: Type-1A angiotensin II receptor
• UniProt Gene Name: Agtr1a
• UniProt Synonym Gene Names: Agtr1; AT1A
• UniProt Entry Name: AGTRA_MOUSE

Uniprot Description

AT1: receptor for angiotensin II. Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. Mediates the major cardiovascular effects of angiotensin II. May play role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1; Receptor, GPCR

Cellular Component: Golgi apparatus; basolateral plasma membrane; cell; dendrite; integral to membrane; endomembrane system; vesicle membrane; cytosol; recycling endosome; membrane; cytoplasm; organelle outer membrane; plasma membrane; cytoplasmic vesicle; endosome

Molecular Function: G-protein coupled receptor activity; signal transducer activity; protein binding; protein self-association; angiotensin type II receptor activity; protein heterodimerization activity; angiotensin type I receptor activity; peptide hormone binding; bradykinin receptor binding; G-protein alpha-subunit binding; protein kinase binding; D1 dopamine receptor binding

Biological Process: activation of JAK protein; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); heart development; drinking behavior; renin-angiotensin regulation of aldosterone production; positive regulation of cellular protein metabolic process; dopamine biosynthetic process; signal transduction; Rho protein signal transduction; elevation of cytosolic calcium ion concentration; regulation of systemic arterial blood pressure by circulatory renin-angiotensin; renin secretion into blood stream; positive regulation of superoxide release; positive regulation of cell proliferation; regulation of pH; negative regulation of neuron apoptosis; inflammatory response; kidney development; positive regulation of receptor recycling; positive regulation of phospholipase A2 activity; blood vessel development; vasoconstriction; angiotensin mediated regulation of renal output; calcium-mediated signaling; brain renin-angiotensin system; G-protein coupled receptor protein signaling pathway; response to estrogen stimulus; regulation of vasoconstriction; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of blood pressure; positive regulation of cytokine secretion