AGTPBP1 sirna

AGTPBP1 siRNA (Mouse)

Cat. Num. AAA8201311

• Gene Names: Agtpbp1; pcd; CCP1; Nna1; nmf243; 1700020N17Rik; 2310001G17Rik; 2900054O13Rik; 4930445M19Rik; 5730402G09Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AGTPBP1; AGTPBP1 siRNA (Mouse); CCP1; NNA1; Cytosolic carboxypeptidase 1; ATP/GTP-binding protein 1; Nervous system nuclear protein induced by axotomy protein 1; AGTPBP1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: AGTPBP1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 688

• Applicable Applications for AGTPBP1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse AGTPBP1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AGTPBP1 sirna

siRNA to inhibit AGTPBP1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 114158683
• NCBI GeneID: 67269
• NCBI Accession #: NP_001041473.1
• NCBI GenBank Nucleotide #: NM_001048008.2
• UniProt Accession #: Q641K1; Q3TDS0; Q3V147; Q6P9R9; Q8C1K8; Q9D962; Q9EQI4
• Molecular Weight: 76,297 Da
• NCBI Official Full Name: cytosolic carboxypeptidase 1 isoform 2
• NCBI Official Synonym Full Names: ATP/GTP binding protein 1
• NCBI Official Symbol: Agtpbp1
• NCBI Official Synonym Symbols: pcd; CCP1; Nna1; nmf243; 1700020N17Rik; 2310001G17Rik; 2900054O13Rik; 4930445M19Rik; 5730402G09Rik
• NCBI Protein Information: cytosolic carboxypeptidase 1
• UniProt Protein Name: Cytosolic carboxypeptidase 1
• UniProt Gene Name: Agtpbp1
• UniProt Synonym Gene Names: Ccp1; Nna1
• UniProt Entry Name: CBPC1_MOUSE

Uniprot Description

Nna1: Metallocarboxypeptidase that mediates deglutamylation of target proteins. Catalyzes the deglutamylation of polyglutamate side chains generated by post-translational polyglutamylation in proteins such as tubulins. Also removes gene-encoded polyglutamates from the carboxy-terminus of target proteins such as MYLK. Acts as a long-chain deglutamylase and specifically shortens long polyglutamate chains, while it is not able to remove the branching point glutamate, a process catalyzed by AGBL5/CCP5. Interacts with MYLK. Belongs to the peptidase M14 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.17.-; Protease

Cellular Component: mitochondrion; cytoplasm; cytosol; nucleus

Molecular Function: tubulin binding; peptidase activity; protein binding; carboxypeptidase activity; zinc ion binding; hydrolase activity; metallopeptidase activity; metal ion binding; metallocarboxypeptidase activity

Biological Process: mitochondrion organization and biogenesis; neurotransmitter metabolic process; retina development in camera-type eye; olfactory bulb development; neuromuscular process; cerebellar Purkinje cell differentiation; cerebellar Purkinje cell layer development; cerebellum development; proteolysis; eye photoreceptor cell differentiation; adult walking behavior