AGK sirna

AGK siRNA (Mouse)

Cat. Num. AAA8233799

• Gene Names: Agk; Mulk; AI465370; 2610037M15Rik; 6720408I04Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AGK; AGK siRNA (Mouse); MULK; Acylglycerol kinase mitochondrial; Multiple substrate lipid kinase; MuLK; Multi-substrate lipid kinase; AGK sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: AGK siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 421

• Applicable Applications for AGK sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse AGK gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AGK sirna

siRNA to inhibit AGK expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 37537518
• NCBI GeneID: 69923
• NCBI Accession #: NP_076027.1
• NCBI GenBank Nucleotide #: NM_023538.2
• UniProt Accession #: Q9ESW4; Q9D087
• Molecular Weight: 18,899 Da
• NCBI Official Full Name: acylglycerol kinase, mitochondrial
• NCBI Official Synonym Full Names: acylglycerol kinase
• NCBI Official Symbol: Agk
• NCBI Official Synonym Symbols: Mulk; AI465370; 2610037M15Rik; 6720408I04Rik
• NCBI Protein Information: acylglycerol kinase, mitochondrial
• UniProt Protein Name: Acylglycerol kinase, mitochondrial
• Protein Family: Acylglycerol kinase
• UniProt Gene Name: Agk
• UniProt Synonym Gene Names: Mulk; MuLK; Multi-substrate lipid kinase
• UniProt Entry Name: AGK_MOUSE

Uniprot Description

AGK: Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively. Does not phosphorylate sphingosine. Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth. Defects in AGK are the cause of mitochondrial DNA depletion syndrome type 10 (MTDPS10). An autosomal recessive mitochondrial disorder characterized by congenital cataracts, hypertrophic cardiomyopathy, skeletal myopathy, exercise intolerance, and lactic acidosis. Mental development is normal, but affected individuals may die early from cardiomyopathy. Defects in AGK are the cause of cataract, congenital, autosomal recessive type 5 (CATC5). CATC5 consists of an opacification of the crystalline lens of the eye becoming evident at birth. It frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.1.107; Motility/polarity/chemotaxis; Lipid Metabolism - glycerolipid; EC 2.7.1.94; Kinase, lipid

Cellular Component: membrane; intracellular membrane-bound organelle; mitochondrion

Molecular Function: transferase activity; lipid kinase activity; acylglycerol kinase activity; ceramide kinase activity; nucleotide binding; kinase activity; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity

Biological Process: metabolic process; protein kinase C activation; ceramide biosynthetic process; lipid phosphorylation; phosphorylation