AFF2 sirna

AFF2 siRNA (Mouse)

Cat. Num. AAA8218207

• Gene Names: Aff2; Oxh; Fmr2; Ox19; FMR2P
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AFF2; AFF2 siRNA (Mouse); FMR2; OX19; AF4/FMR2 family member 2; FMR2P; Fragile X mental retardation protein 2 homolog; Protein FMR-2; Protein Ox19; AFF2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: AFF2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1272

• Applicable Applications for AFF2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse AFF2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AFF2 sirna

siRNA to inhibit AFF2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 153792808
• NCBI GeneID: 14266
• NCBI Accession #: NP_032058.2
• NCBI GenBank Nucleotide #: NM_008032.3
• UniProt Accession #: O55112; B1ATW0
• Molecular Weight: 140,181 Da
• NCBI Official Full Name: AF4/FMR2 family member 2
• NCBI Official Synonym Full Names: AF4/FMR2 family, member 2
• NCBI Official Symbol: Aff2
• NCBI Official Synonym Symbols: Oxh; Fmr2; Ox19; FMR2P
• NCBI Protein Information: AF4/FMR2 family member 2
• UniProt Protein Name: AF4/FMR2 family member 2
• Protein Family: AF4/FMR2 family
• UniProt Gene Name: Aff2
• UniProt Synonym Gene Names: Fmr2; Ox19; Protein FMR-2
• UniProt Entry Name: AFF2_MOUSE

Uniprot Description

AFF2: RNA-binding protein. Might be involved in alternative splicing regulation through an interaction with G-quartet RNA structure. Defects in AFF2 are the cause of mental retardation X- linked associated with fragile site FRAXE (MRFRAXE). A form of mild to moderate mental retardation associated with learning difficulties, communication deficits, attention problems, hyperactivity, and autistic behavior. It is associated with a fragile site on chromosome Xq28. Mental retardation is characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Caused either by silencing of the AFF2 gene as a consequence of a CCG expansion located upstream of this gene or by deletion within the gene. Loss of AFF2 expression is correlated with FRAXE CCG(N) expansion. Normal individuals have 6-35 copies of the repeat, whereas cytogenetically positive, developmentally delayed males have more than 200 copies and show methylation of the associated CPG island. Belongs to the AF4 family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Cellular Component: nuclear speck; nucleus

Molecular Function: RNA binding

Biological Process: learning and/or memory; regulation of RNA splicing; RNA splicing; mRNA processing