ADPRHL2 sirna

ADPRHL2 siRNA (Mouse)

Cat. Num. AAA8219616

• Gene Names: Adprhl2; Arh3; AI836109
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ADPRHL2; ADPRHL2 siRNA (Mouse); ARH3; Poly(ADP-ribose) glycohydrolase ARH3; ADP-ribosylhydrolase 3; [Protein ADP-ribosylarginine] hydrolase-like protein 2; ADPRHL2 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ADPRHL2 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 370

• Applicable Applications for ADPRHL2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ADPRHL2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ADPRHL2 sirna

siRNA to inhibit ADPRHL2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 28916671
• NCBI GeneID: 100206
• NCBI Accession #: NP_598644.1
• NCBI GenBank Nucleotide #: NM_133883.2
• UniProt Accession #: Q8CG72; Q80UW9; Q8R575; Q921U6; A3KFY3
• Molecular Weight: 31,043 Da
• NCBI Official Full Name: poly(ADP-ribose) glycohydrolase ARH3
• NCBI Official Synonym Full Names: ADP-ribosylhydrolase like 2
• NCBI Official Symbol: Adprhl2
• NCBI Official Synonym Symbols: Arh3; AI836109
• NCBI Protein Information: poly(ADP-ribose) glycohydrolase ARH3
• UniProt Protein Name: Poly(ADP-ribose) glycohydrolase ARH3
• Protein Family: Poly(ADP-ribose) glycohydrolase
• UniProt Gene Name: Adprhl2
• UniProt Synonym Gene Names: Arh3
• UniProt Entry Name: ARHL2_MOUSE

Uniprot Description

ADPRHL2: Poly(ADP-ribose) synthesized after DNA damage is only present transiently and is rapidly degraded by poly(ADP-ribose) glycohydrolase. Poly(ADP-ribose) metabolism may be required for maintenance of the normal function of neuronal cells. Generates ADP-ribose from poly-(ADP-ribose), but does not hydrolyze ADP- ribose-arginine, -cysteine, -diphthamide, or -asparagine bonds. Belongs to the ADP-ribosylglycohydrolase family.

Protein type: Hydrolase; EC 3.2.1.143

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleus

Molecular Function: hydrolase activity; metal ion binding; poly(ADP-ribose) glycohydrolase activity