ADAM17 sirna

ADAM17 siRNA (Mouse)

Cat. Num. AAA8212119

• Gene Names: Adam17; Tace; CD156b
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ADAM17; ADAM17 siRNA (Mouse); TACE; Disintegrin and metalloproteinase domain-containing protein 17; ADAM 17; TNF-alpha convertase; TNF-alpha-converting enzyme; CD antigen CD156b; ADAM17 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ADAM17 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 846

• Applicable Applications for ADAM17 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ADAM17 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ADAM17 sirna

siRNA to inhibit ADAM17 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 471270257
• NCBI GeneID: 11491
• NCBI Accession #: NP_001264195.1
• NCBI GenBank Nucleotide #: NM_001277266.1
• UniProt Accession #: Q9Z0F8; O88726; Q505A7; Q9R1U4; Q9Z0K3
• Molecular Weight: 73,884 Da
• NCBI Official Full Name: disintegrin and metalloproteinase domain-containing protein 17 isoform 2
• NCBI Official Synonym Full Names: a disintegrin and metallopeptidase domain 17
• NCBI Official Symbol: Adam17
• NCBI Official Synonym Symbols: Tace; CD156b
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 17
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 17
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: Adam17
• UniProt Synonym Gene Names: Tace; ADAM 17
• UniProt Entry Name: ADA17_MOUSE

Uniprot Description

TACE: a type I membrane protein with disintegrin and metalloprotease activity. An ubiquitously expressed ectoenzyme of peptidase family M12B. Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Possesses a narrow endopeptidase specificity. Cleaves Pro-Leu-Ala-Gln-Ala-|-Val-Arg-Ser-Ser-Ser in the membrane-bound, 26-kDa form of tumor necrosis factor alpha (TNF-alpha) to its mature soluble form. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, and the amyloid precursor protein. Also involved in the activation of Notch pathway. Binds 1 zinc ion per subunit. Two splice variant isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Protease; EC 3.4.24.86

Cellular Component: cell surface; focal adhesion; membrane; integral to plasma membrane; apical plasma membrane; cytoplasm; integral to membrane; extracellular region; plasma membrane; intercellular junction; actin cytoskeleton; lipid raft

Molecular Function: integrin binding; peptidase activity; protein binding; interleukin-6 receptor binding; zinc ion binding; hydrolase activity; metallopeptidase activity; metalloendopeptidase activity; metal ion binding; Notch binding; SH3 domain binding; PDZ domain binding

Biological Process: T cell differentiation in the thymus; cell motility involved in cell locomotion; response to lipopolysaccharide; proteolysis; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; positive regulation of epidermal growth factor receptor activity; positive regulation of cell proliferation; germinal center formation; negative regulation of interleukin-8 production; cell adhesion; epidermal growth factor receptor signaling pathway; response to drug; spleen development; Notch signaling pathway; response to high density lipoprotein stimulus; membrane protein ectodomain proteolysis; positive regulation of transforming growth factor beta receptor signaling pathway; Notch receptor processing; positive regulation of cell motility; positive regulation of cell growth; positive regulation of chemokine production; regulation of mast cell apoptosis; PMA-inducible membrane protein ectodomain proteolysis; B cell differentiation; response to hypoxia; cell adhesion mediated by integrin; positive regulation of protein amino acid phosphorylation; negative regulation of transforming growth factor beta receptor signaling pathway; negative regulation of apoptosis; positive regulation of cell migration