ACOX1 sirna

ACOX1 siRNA (Mouse)

Cat. Num. AAA8225192

• Gene Names: Acox1; AOX; Acox; Paox; D130055E20Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ACOX1; ACOX1 siRNA (Mouse); ACOX; PAOX; Peroxisomal acyl-coenzyme A oxidase 1; AOX; Palmitoyl-CoA oxidase; ACOX1 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ACOX1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 661

• Applicable Applications for ACOX1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ACOX1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ACOX1 sirna

siRNA to inhibit ACOX1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 429484484
• NCBI GeneID: 11430
• NCBI Accession #: NP_001258827.1
• NCBI GenBank Nucleotide #: NM_001271898.1
• UniProt Accession #: Q9R0H0; O35616; Q3TDG0; Q8BYC3; A2A850
• Molecular Weight: 74,718 Da
• NCBI Official Full Name: peroxisomal acyl-coenzyme A oxidase 1 isoform 2
• NCBI Official Synonym Full Names: acyl-Coenzyme A oxidase 1, palmitoyl
• NCBI Official Symbol: Acox1
• NCBI Official Synonym Symbols: AOX; Acox; Paox; D130055E20Rik
• NCBI Protein Information: peroxisomal acyl-coenzyme A oxidase 1
• UniProt Protein Name: Peroxisomal acyl-coenzyme A oxidase 1
• Protein Family: Peroxisomal acyl-coenzyme A oxidase
• UniProt Gene Name: Acox1
• UniProt Synonym Gene Names: Acox; Paox; AOX
• UniProt Entry Name: ACOX1_MOUSE

NCBI Description

This gene encodes a member of the acyl-coenzyme A oxidase family. The encoded protein is localized to peroxisomes and is the first enzyme of the fatty acid beta-oxidation pathway, which catalyzes the desaturation of acyl-coenzyme A to 2-trans-enoyl-coenzyme A. Disruption of this gene results in microvesicular steatohepatitis, spontaneous peroxisome proliferation, and the eventual development of hepatocellular carcinomas. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012]

Uniprot Description

ACOX1: Catalyzes the desaturation of acyl-CoAs to 2-trans- enoyl-CoAs. Isoform 1 shows highest activity against medium-chain fatty acyl-CoAs and activity decreases with increasing chain length. Isoform 2 is active against a much broader range of substrates and shows activity towards very long-chain acyl-CoAs. Isoform 2 is twice as active as isoform 1 against 16-hydroxy- palmitoyl-CoA and is 25% more active against 1,16-hexadecanodioyl- CoA. Defects in ACOX1 are the cause of adrenoleukodystrophy pseudoneonatal (Pseudo-NALD); also known as peroxisomal acyl-CoA oxidase deficiency. Pseudo-NALD is a peroxisomal single-enzyme disorder. Clinical features include mental retardation, leukodystrophy, seizures, mild hepatomegaly, hearing deficit. Pseudo-NALD is characterized by increased plasma levels of very-long chain fatty cids, due to decreased or absent peroxisome acyl-CoA oxidase activity. Peroxisomes are intact and functioning. Belongs to the acyl-CoA oxidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - fatty acid; Lipid Metabolism - alpha-linolenic acid; Lipid Metabolism - unsaturated fatty acid biosynthesis; EC 1.3.3.6; Oxidoreductase

Cellular Component: nucleoplasm; peroxisomal membrane; mitochondrion; membrane; intracellular membrane-bound organelle; cytoplasm; nucleolus; plasma membrane; peroxisome; nucleus

Molecular Function: palmitoyl-CoA oxidase activity; acyl-CoA dehydrogenase activity; acyl-CoA oxidase activity; FAD binding; oxidoreductase activity, acting on the CH-CH group of donors; protein N-terminus binding; oxidoreductase activity; fatty acid binding; PDZ domain binding; receptor binding

Biological Process: peroxisome fission; fatty acid beta-oxidation using acyl-CoA oxidase; generation of precursor metabolites and energy; fatty acid beta-oxidation; metabolic process; very-long-chain fatty acid metabolic process; lipid homeostasis; spermatogenesis; fatty acid oxidation; lipid metabolic process; fatty acid metabolic process; prostaglandin metabolic process