ACOT7 sirna

ACOT7 siRNA (Mouse)

Cat. Num. AAA8218570

• Gene Names: Acot7; Act; Ach1; Bach; Lach1; Cte-II; CTE-IIa; 2410041A17Rik
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ACOT7; ACOT7 siRNA (Mouse); BACH; Cytosolic acyl coenzyme A thioester hydrolase; Acyl-CoA thioesterase 7; Brain acyl-CoA hydrolase; CTE-IIa; CTE-II; Long chain acyl-CoA thioester hydrolase; ACOT7 sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ACOT7 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 384

• Applicable Applications for ACOT7 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ACOT7 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ACOT7 sirna

siRNA to inhibit ACOT7 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 225690616
• NCBI GeneID: 70025
• NCBI Accession #: NP_001139529.1
• NCBI GenBank Nucleotide #: NM_001146057.1
• UniProt Accession #: Q91V12; Q3U8C6; Q59HQ2; A2A8K9; A2A8L0; A2A8L2
• Molecular Weight: 41,616 Da
• NCBI Official Full Name: cytosolic acyl coenzyme A thioester hydrolase isoform 1
• NCBI Official Synonym Full Names: acyl-CoA thioesterase 7
• NCBI Official Symbol: Acot7
• NCBI Official Synonym Symbols: Act; Ach1; Bach; Lach1; Cte-II; CTE-IIa; 2410041A17Rik
• NCBI Protein Information: cytosolic acyl coenzyme A thioester hydrolase
• UniProt Protein Name: Cytosolic acyl coenzyme A thioester hydrolase
• Protein Family: Cytosolic acyl coenzyme A thioester hydrolase
• UniProt Gene Name: Acot7
• UniProt Synonym Gene Names: Bach; BACH; CTE-II
• UniProt Entry Name: BACH_MOUSE

Uniprot Description

ACOT7: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. May play an important physiological function in brain. May play a regulatory role by modulating the cellular levels of fatty acyl- CoA ligands for certain transcription factors as well as the substrates for fatty acid metabolizing enzymes, contributing to lipid homeostasis. Has broad specificity, active towards fatty acyl-CoAs with chain-lengths of C8-C18. Has a maximal activity toward palmitoyl-CoA. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; EC 3.1.2.2; Lipid Metabolism - unsaturated fatty acid biosynthesis

Cellular Component: nucleoplasm; neuron projection; cytoplasm; cytosol

Molecular Function: acyl-CoA hydrolase activity; protein homodimerization activity; acyl-CoA binding; hydrolase activity; palmitoyl-CoA hydrolase activity

Biological Process: medium-chain fatty acid biosynthetic process; coenzyme A biosynthetic process; fatty acid catabolic process