ACADVL sirna

ACADVL siRNA (Mouse)

Cat. Num. AAA8204357

• Gene Names: Acadvl; vlcad
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ACADVL; ACADVL siRNA (Mouse); VLCAD; Very long-chain specific acyl-CoA dehydrogenase mitochondrial; MVLCAD; ACADVL sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: ACADVL siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 656

• Applicable Applications for ACADVL sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse ACADVL gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ACADVL sirna

siRNA to inhibit ACADVL expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 23956084
• NCBI GeneID: 11370
• NCBI Accession #: NP_059062.1
• NCBI GenBank Nucleotide #: NM_017366.3
• UniProt Accession #: P50544; O35289; O55133
• Molecular Weight: 70,875 Da
• NCBI Official Full Name: very long-chain specific acyl-CoA dehydrogenase, mitochondrial
• NCBI Official Synonym Full Names: acyl-Coenzyme A dehydrogenase, very long chain
• NCBI Official Symbol: Acadvl
• NCBI Official Synonym Symbols: vlcad
• NCBI Protein Information: very long-chain specific acyl-CoA dehydrogenase, mitochondrial
• UniProt Protein Name: Very long-chain specific acyl-CoA dehydrogenase, mitochondrial
• Protein Family: Very long-chain specific acyl-CoA dehydrogenase
• UniProt Gene Name: Acadvl
• UniProt Synonym Gene Names: Vlcad; VLCAD
• UniProt Entry Name: ACADV_MOUSE

NCBI Description

This gene encodes a homodimeric mitochondrial flavoprotein and is a member of the acyl-CoA dehydrogenase family. Members of this family catalyze the first step of fatty acid beta-oxidation, forming a C2-C3 trans-double bond in a FAD-dependent reaction. As beta-oxidation cycles through its four steps, each member of the acyl-CoA dehydrogenase family works at an optimum fatty acid chain-length. This enzyme has its optimum length between C16- and C20-acylCoA and localizes to the inner mitochondrial membrane (unlike related acyl-CoA dehydrogenases). In mice, deficiency of this gene can cause ventricular arrhythmias as well as fasting and cold intolerance. [provided by RefSeq, Nov 2012]

Uniprot Description

ACADVL: Active toward esters of long-chain and very long chain fatty acids such as palmitoyl-CoA, mysritoyl-CoA and stearoyl-CoA. Can accommodate substrate acyl chain lengths as long as 24 carbons, but shows little activity for substrates of less than 12 carbons. Defects in ACADVL are the cause of acyl-CoA dehydrogenase very long chain deficiency (ACADVLD). ACADVLD is an autosomal recessive disease which leads to impaired long-chain fatty acid beta-oxidation. It is clinically heterogeneous, with three major phenotypes: a severe childhood form, with early onset, high mortality, and high incidence of cardiomyopathy; a milder childhood form, with later onset, usually with hypoketotic hypoglycemia as the main presenting feature, low mortality, and rare cardiomyopathy; and an adult form, with isolated skeletal muscle involvement, rhabdomyolysis, and myoglobinuria, usually triggered by exercise or fasting. Belongs to the acyl-CoA dehydrogenase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - fatty acid; Mitochondrial; Oxidoreductase; EC 1.3.8.9

Cellular Component: membrane; mitochondrion; cytoplasm; mitochondrial inner membrane; nucleolus; nucleus

Molecular Function: acyl-CoA dehydrogenase activity; FAD binding; acyl-CoA binding; very-long-chain-acyl-CoA dehydrogenase activity; oxidoreductase activity, acting on the CH-CH group of donors; oxidoreductase activity; long-chain-acyl-CoA dehydrogenase activity

Biological Process: fatty acid beta-oxidation; metabolic process; very-long-chain fatty acid catabolic process; lipid metabolic process; thermoregulation; fatty acid metabolic process; fatty acid beta-oxidation using acyl-CoA dehydrogenase; negative regulation of fatty acid oxidation; negative regulation of fatty acid biosynthetic process