AADAT sirna

AADAT siRNA (Mouse)

Cat. Num. AAA8221814

• Gene Names: Aadat; Aadt; Kat2; KATII; mKat-2; AI875679
• Reactivity: Mouse
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: AADAT; AADAT siRNA (Mouse); KAT2; Kynurenine/alpha-aminoadipate aminotransferase mitochondrial; KAT/AadAT; 2-aminoadipate aminotransferase; 2-aminoadipate transaminase; Alpha-aminoadipate aminotransferase; AadAT; Kynurenine aminotransferase II; Kynurenine--oxoglutarate aminotransferase II; Kynurenine--oxoglutarate transaminase 2; Kynurenine--oxoglutarate transaminase II; AADAT sirna

• Host: Synthetic
• Reactivity: Mouse
• Specificity: AADAT siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 425

• Applicable Applications for AADAT sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse AADAT gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for AADAT sirna

siRNA to inhibit AADAT expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 6754408
• NCBI GeneID: 23923
• NCBI Accession #: NP_035964.1
• NCBI GenBank Nucleotide #: NM_011834.2
• UniProt Accession #: Q9WVM8
• Molecular Weight: 47,598 Da
• NCBI Official Full Name: kynurenine/alpha-aminoadipate aminotransferase, mitochondrial
• NCBI Official Synonym Full Names: aminoadipate aminotransferase
• NCBI Official Symbol: Aadat
• NCBI Official Synonym Symbols: Aadt; Kat2; KATII; mKat-2; AI875679
• NCBI Protein Information: kynurenine/alpha-aminoadipate aminotransferase, mitochondrial
• UniProt Protein Name: Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial
• Protein Family: Kynurenine/alpha-aminoadipate aminotransferase
• UniProt Gene Name: Aadat
• UniProt Synonym Gene Names: Kat2; KAT/AadAT; AadAT
• UniProt Entry Name: AADAT_MOUSE

Uniprot Description

AADAT: Transaminase with broad substrate specificity. Has transaminase activity towards aminoadipate, kynurenine, methionine and glutamate. Shows activity also towards tryptophan, aspartate and hydroxykynurenine. Accepts a variety of oxo-acids as amino- group acceptors, with a preference for 2-oxoglutarate, 2- oxocaproic acid, phenylpyruvate and alpha-oxo-gamma-methiol butyric acid. Can also use glyoxylate as amino-group acceptor (in vitro). Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - lysine biosynthesis; Mitochondrial; EC 2.6.1.39; Amino Acid Metabolism - lysine degradation; EC 2.6.1.7; Amino Acid Metabolism - tryptophan; Transferase

Cellular Component: mitochondrion

Molecular Function: transferase activity; protein homodimerization activity; kynurenine-oxoglutarate transaminase activity; transaminase activity; 2-aminoadipate transaminase activity; catalytic activity; pyridoxal phosphate binding

Biological Process: glutamate metabolic process; tryptophan catabolic process to kynurenine; biosynthetic process; 2-oxoglutarate metabolic process