ZP2 sirna

ZP2 siRNA (Human)

Cat. Num. AAA8230471

• Gene Names: ZP2; ZPA; Zp-2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ZP2; ZP2 siRNA (Human); ZPA; Zona pellucida sperm-binding protein 2; Zona pellucida glycoprotein 2; Zp-2; Zona pellucida protein A; ZP2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ZP2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 736

• Applicable Applications for ZP2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ZP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ZP2 sirna

siRNA to inhibit ZP2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 588282802
• NCBI GeneID: 7783
• NCBI Accession #: NP_001277033.1
• NCBI GenBank Nucleotide #: NM_001290104.1
• UniProt Accession #: Q05996; Q4VAN9; Q4VAP0; Q4VAP1; B2R7J2
• Molecular Weight: 81,300 Da
• NCBI Official Full Name: zona pellucida sperm-binding protein 2 isoform 2 preproprotein
• NCBI Official Synonym Full Names: zona pellucida glycoprotein 2 (sperm receptor)
• NCBI Official Symbol: ZP2
• NCBI Official Synonym Symbols: ZPA; Zp-2
• NCBI Protein Information: zona pellucida sperm-binding protein 2
• UniProt Protein Name: Zona pellucida sperm-binding protein 2
• Protein Family: Zona pellucida sperm-binding protein
• UniProt Gene Name: ZP2
• UniProt Synonym Gene Names: ZPA; Zp-2
• UniProt Entry Name: ZP2_HUMAN

NCBI Description

The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed of three glycoproteins with various functions during fertilization and preimplantation development. The glycosylated mature peptide is one of the structural components of the zona pellucida and functions in secondary binding and penetration of acrosome-reacted spermatozoa. Female mice lacking this gene do not form a stable zona matrix and are sterile. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Uniprot Description

ZP2: The mammalian zona pellucida, which mediates species- specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP2 may act as a secondary sperm receptor. Belongs to the ZP domain family. ZPA subfamily.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 16p12

Cellular Component: extracellular matrix; multivesicular body; Golgi apparatus; proteinaceous extracellular matrix; endoplasmic reticulum; plasma membrane; integral to membrane; extracellular region; secretory granule

Molecular Function: acrosin binding; coreceptor activity

Biological Process: intracellular protein transport; binding of sperm to zona pellucida; single fertilization; manganese ion transport; regulation of acrosome reaction; signal transduction; multicellular organism reproduction