ZIC2 sirna

ZIC2 siRNA (Human)

Cat. Num. AAA8238517

• Gene Names: ZIC2; HPE5
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ZIC2; ZIC2 siRNA (Human); Zinc finger protein ZIC 2; Zinc finger protein of the cerebellum 2; ZIC2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ZIC2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 532

• Applicable Applications for ZIC2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ZIC2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ZIC2 sirna

siRNA to inhibit ZIC2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 22547197
• NCBI GeneID: 7546
• NCBI Accession #: NP_009060.2
• NCBI GenBank Nucleotide #: NM_007129.3
• UniProt Accession #: O95409; Q5VYA9; Q9H309
• Molecular Weight: 55,006 Da
• NCBI Official Full Name: zinc finger protein ZIC 2
• NCBI Official Synonym Full Names: Zic family member 2
• NCBI Official Symbol: ZIC2
• NCBI Official Synonym Symbols: HPE5
• NCBI Protein Information: zinc finger protein ZIC 2
• UniProt Protein Name: Zinc finger protein ZIC 2
• Protein Family: Zinc finger protein
• UniProt Gene Name: ZIC2
• UniProt Entry Name: ZIC2_HUMAN

NCBI Description

This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. This protein functions as a transcriptional repressor and may regulate tissue specific expression of dopamine receptor D1. Mutations in this gene cause holoprosencephaly type 5. Holoprosencephaly is the most common structural anomaly of the human brain. A polyhistidine tract polymorphism in this gene may be associated with increased risk of neural tube defects. This gene is closely linked to a gene encoding zinc finger protein of the cerebellum 5, a related family member on chromosome 13. [provided by RefSeq, Jul 2008]

Uniprot Description

ZIC2: Acts as a transcriptional activator or repressor. Plays important roles in the early stage of organogenesis of the CNS. Activates the transcription of the serotonin transporter SERT in uncrossed ipsilateral retinal ganglion cells (iRGCs) to refine eye-specific projections in primary visual targets. Its transcriptional activity is repressed by MDFIC. Involved in the formation of the ipsilateral retinal projection at the optic chiasm midline. Drives the expression of EPHB1 on ipsilaterally projecting growth cones. Binds to the minimal GLI-consensus sequence 5'-TGGGTGGTC-3'. Associates to the basal SERT promoter region from ventrotemporal retinal segments of retinal embryos. Defects in ZIC2 are a cause of holoprosencephaly type 5 (HPE5). A structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability. Although severe facial anomalies are common in HPE, patients with ZINC2 mutations have relatively normal faces. Belongs to the GLI C2H2-type zinc-finger protein family.

Protein type: DNA-binding; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 13q32

Cellular Component: cytoplasm; nucleus

Molecular Function: DNA binding; chromatin DNA binding; metal ion binding; transcription factor activity

Biological Process: visual perception; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; pigmentation during development; neural tube closure; positive regulation of transcription factor activity; brain development; negative regulation of transcription, DNA-dependent; retinal ganglion cell axon guidance

Disease: Holoprosencephaly 5