ZFPM1 sirna

ZFPM1 siRNA (Human)

Cat. Num. AAA8236448

• Gene Names: ZFPM1; FOG; FOG1; ZNF408; ZNF89A; ZC2HC11A
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ZFPM1; ZFPM1 siRNA (Human); FOG1; ZFN89A; Zinc finger protein ZFPM1; Friend of GATA protein 1; FOG-1; Friend of GATA 1; Zinc finger protein 89A; Zinc finger protein multitype 1; ZFPM1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ZFPM1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 1,006

• Applicable Applications for ZFPM1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ZFPM1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ZFPM1 sirna

siRNA to inhibit ZFPM1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 110556644
• NCBI GeneID: 161882
• NCBI Accession #: NP_722520.2
• NCBI GenBank Nucleotide #: NM_153813.2
• UniProt Accession #: Q8IX07
• Molecular Weight: 104,888 Da
• NCBI Official Full Name: zinc finger protein ZFPM1
• NCBI Official Synonym Full Names: zinc finger protein, FOG family member 1
• NCBI Official Symbol: ZFPM1
• NCBI Official Synonym Symbols: FOG; FOG1; ZNF408; ZNF89A; ZC2HC11A
• NCBI Protein Information: zinc finger protein ZFPM1
• UniProt Protein Name: Zinc finger protein ZFPM1
• Protein Family: Zinc finger protein
• UniProt Gene Name: ZFPM1
• UniProt Synonym Gene Names: FOG1; ZFN89A; FOG-1; Friend of GATA 1
• UniProt Entry Name: FOG1_HUMAN

Uniprot Description

FOG1: Transcription regulator that plays an essential role in erythroid and megakaryocytic cell differentiation. Essential cofactor that acts via the formation of a heterodimer with transcription factors of the GATA family GATA1, GATA2 and GATA3. Such heterodimer can both activate or repress transcriptional activity, depending on the cell and promoter context. The heterodimer formed with GATA proteins is essential to activate expression of genes such as NFE2, ITGA2B, alpha- and beta-globin, while it represses expression of KLF1. May be involved in regulation of some genes in gonads. May also be involved in cardiac development, in a non-redundant way with ZFPM2/FOG2. Belongs to the FOG (Friend of GATA) family.

Protein type: C2H2-type zinc finger protein; Transcription regulation

Chromosomal Location of Human Ortholog: 16q24.2

Cellular Component: nucleoplasm; transcription factor complex; transcriptional repressor complex; cytoplasm; nucleus

Molecular Function: protein binding; DNA binding; metal ion binding; transcription factor binding

Biological Process: transcription, DNA-dependent; granulocyte differentiation; megakaryocyte differentiation; embryonic hemopoiesis; heart development; T-helper cell lineage commitment; negative regulation of transcription from RNA polymerase II promoter; negative regulation of fat cell differentiation; negative regulation of interleukin-4 biosynthetic process; cardiac muscle morphogensis; erythrocyte differentiation; platelet formation; blood coagulation; negative regulation of protein binding; regulation of chemokine production; positive regulation of interferon-gamma biosynthetic process