ZBED1 sirna

ZBED1 siRNA (Human)

Cat. Num. AAA8201477

• Gene Names: ZBED1; ALTE; DREF; TRAMP; hDREF
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ZBED1; ZBED1 siRNA (Human); ALTE; DREF; KIAA0785; TRAMP; Zinc finger BED domain-containing protein 1; Putative Ac-like transposable element; dREF homolog; ZBED1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ZBED1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 694

• Applicable Applications for ZBED1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ZBED1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ZBED1 sirna

siRNA to inhibit ZBED1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 283806699
• NCBI GeneID: 9189
• NCBI Accession #: NP_001164606.1
• NCBI GenBank Nucleotide #: NM_001171135.1
• UniProt Accession #: O96006; Q96BY4
• Molecular Weight: 78,156 Da
• NCBI Official Full Name: zinc finger BED domain-containing protein 1
• NCBI Official Synonym Full Names: zinc finger, BED-type containing 1
• NCBI Official Symbol: ZBED1
• NCBI Official Synonym Symbols: ALTE; DREF; TRAMP; hDREF
• NCBI Protein Information: zinc finger BED domain-containing protein 1
• UniProt Protein Name: Zinc finger BED domain-containing protein 1
• Protein Family: Zinc finger BED domain-containing protein
• UniProt Gene Name: ZBED1
• UniProt Synonym Gene Names: ALTE; DREF; KIAA0785; TRAMP
• UniProt Entry Name: ZBED1_HUMAN

NCBI Description

This gene is located in the pseudoautosomal region 1 (PAR1) of X and Y chromosomes. It was earlier identified as a gene with similarity to Ac transposable elements, however, was found not to have transposase activity. Later studies show that this gene product is localized in the nucleus and functions as a transcription factor. It binds to DNA elements found in the promoter regions of several genes related to cell proliferation, such as histone H1, hence may have a role in regulating genes related to cell proliferation. Alternatively spliced transcript variants with different 5' untranslated region have been found for this gene. [provided by RefSeq, Jan 2010]

Uniprot Description

ZBED1: Binds to 5'-TGTCG[CT]GA[CT]A-3' DNA elements found in the promoter regions of a number of genes related to cell proliferation. Binds to the histone H1 promoter and stimulates transcription. Was first identified as gene weakly similar to Ac transposable elements, but does not code for any transposase activity.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: Xp22.33;Yp11

Cellular Component: nuclear chromosome

Molecular Function: protein dimerization activity; DNA binding; metal ion binding; transposase activity

Biological Process: metabolic process