ZAN sirna

ZAN siRNA (Human)

Cat. Num. AAA8212110

• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: ZAN; ZAN siRNA (Human); Zonadhesin; ZAN sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: ZAN siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 2812

• Applicable Applications for ZAN sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human ZAN gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for ZAN sirna

siRNA to inhibit ZAN expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 603844408
• NCBI GeneID: 7455
• NCBI Accession #: NP_003377.2
• NCBI GenBank Nucleotide #: NM_003386.2
• UniProt Accession #: Q9Y493; O00218; Q96L85; Q96L86; Q96L87; Q96L88; Q96L89; Q96L90; Q9BXN9; Q9BZ83; A0FKC8; D6W5W4
• Molecular Weight: 311,094 Da
• NCBI Official Full Name: zonadhesin isoform 3
• NCBI Official Synonym Full Names: zonadhesin (gene/pseudogene)
• NCBI Official Symbol: ZAN
• NCBI Protein Information: zonadhesin
• UniProt Protein Name: Zonadhesin
• Protein Family: Zonadhesin
• UniProt Gene Name: ZAN
• UniProt Entry Name: ZAN_HUMAN

NCBI Description

This gene encodes a protein that functions in the species specificity of sperm adhesion to the egg zona pellucida. The encoded protein is located in the acrosome and may be involved in signaling or gamete recognition. An allelic polymorphism in this gene results in both functional and frameshifted alleles; the reference genome represents the functional allele. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2015]

Uniprot Description

ZAN: Binds in a species-specific manner to the zona pellucida of the egg. May be involved in gamete recognition and/or signaling. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: plasma membrane; integral to membrane

Biological Process: cell-cell adhesion; binding of sperm to zona pellucida