YBX2 sirna

YBX2 siRNA (Human)

Cat. Num. AAA8212094

• Gene Names: YBX2; DBPC; MSY2; CSDA3; CONTRIN
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: YBX2; YBX2 siRNA (Human); CSDA3; MSY2; Y-box-binding protein 2; Contrin; DNA-binding protein C; Dbpc; Germ cell-specific Y-box-binding protein; MSY2 homolog; YBX2 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: YBX2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 364

• Applicable Applications for YBX2 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human YBX2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for YBX2 sirna

siRNA to inhibit YBX2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 156415990
• NCBI GeneID: 51087
• NCBI Accession #: NP_057066.2
• NCBI GenBank Nucleotide #: NM_015982.3
• UniProt Accession #: Q9Y2T7; Q8N4P0; D3DTP1
• Molecular Weight: 38,518 Da
• NCBI Official Full Name: Y-box-binding protein 2
• NCBI Official Synonym Full Names: Y box binding protein 2
• NCBI Official Symbol: YBX2
• NCBI Official Synonym Symbols: DBPC; MSY2; CSDA3; CONTRIN
• NCBI Protein Information: Y-box-binding protein 2
• UniProt Protein Name: Y-box-binding protein 2
• Protein Family: Y-box-binding protein
• UniProt Gene Name: YBX2
• UniProt Synonym Gene Names: CSDA3; MSY2; Dbpc
• UniProt Entry Name: YBOX2_HUMAN

NCBI Description

This gene encodes a nucleic acid binding protein which is highly expressed in germ cells. The encoded protein binds to a Y-box element in the promoters of certain genes but also binds to mRNA transcribed from these genes. Pseudogenes for this gene are located on chromosome 10 and 15. [provided by RefSeq, Feb 2012]

Uniprot Description

YBX2: Major constituent of messenger ribonucleoprotein particles (mRNPs). Involved in the regulation of the stability and/or translation of germ cell mRNAs. Binds to Y-box consensus promoter element. Binds to full length mRNA with high affinity in a sequence-independent manner. Binds to short RNA sequences containing the consensus site 5'-UCCAUCA-3' with low affinity and limited sequence specificity. Its binding with maternal mRNAs is necessary for its cytoplasmic retention. May mark specific mRNAs (those transcribed from Y-box promoters) in the nucleus for cytoplasmic storage, thereby linking transcription and mRNA storage/translational delay.

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: polysome; cytoplasm; nucleolus; nucleus

Molecular Function: ribonucleoprotein binding; mRNA 3'-UTR binding; DNA binding; translation regulator activity; chromatin binding; lipid binding

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; translational attenuation; negative regulation of translation; mRNA stabilization; spermatogenesis; negative regulation of binding; spermatid development; oocyte development