WNT1 sirna

WNT1 siRNA (Human)

Cat. Num. AAA8237386

• Gene Names: WNT1; INT1; OI15; BMND16
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: WNT1; WNT1 siRNA (Human); INT1; Proto-oncogene Wnt-1; Proto-oncogene Int-1 homolog; WNT1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: WNT1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 370

• Applicable Applications for WNT1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human WNT1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for WNT1 sirna

siRNA to inhibit WNT1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 139743
• NCBI GeneID: 7471
• UniProt Accession #: P04628
• NCBI Official Full Name: Proto-oncogene Wnt-1
• NCBI Official Synonym Full Names: wingless-type MMTV integration site family, member 1
• NCBI Official Symbol: WNT1
• NCBI Official Synonym Symbols: INT1; OI15; BMND16
• NCBI Protein Information: proto-oncogene Wnt-1
• UniProt Protein Name: Proto-oncogene Wnt-1
• Protein Family: WNT1-inducible-signaling pathway protein
• UniProt Gene Name: WNT1
• UniProt Synonym Gene Names: INT1

NCBI Description

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. This gene is clustered with another family member, WNT10B, in the chromosome 12q13 region. [provided by RefSeq, Jul 2008]

Uniprot Description

WNT1: Ligand for members of the frizzled family of seven transmembrane receptors. In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling. Probable developmental protein. May be a signaling molecule important in CNS development. Is likely to signal over only few cell diameters. Belongs to the Wnt family.

Protein type: Cell development/differentiation; Secreted, signal peptide; Motility/polarity/chemotaxis; Oncoprotein; Secreted

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: proteinaceous extracellular matrix; extracellular space; cell surface; endoplasmic reticulum lumen; Golgi lumen; cytoplasm; plasma membrane; extracellular region

Molecular Function: protein domain specific binding; frizzled binding; cytokine activity; receptor agonist activity

Biological Process: positive regulation of insulin-like growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; T cell differentiation in the thymus; Wnt receptor signaling pathway through beta-catenin; ubiquitin-dependent SMAD protein catabolic process; diencephalon development; negative regulation of BMP signaling pathway; neuron differentiation; cerebellum formation; positive regulation of fibroblast proliferation; cell-cell signaling; midbrain development; positive regulation of cell proliferation; midbrain-hindbrain boundary maturation during brain development; response to wounding; positive regulation of Notch signaling pathway; myoblast fusion; neuron fate determination; organ regeneration; inner ear morphogenesis; Wnt receptor signaling pathway; cell fate commitment; embryonic axis specification; DNA damage response, signal transduction; negative regulation of fat cell differentiation; central nervous system morphogenesis; ureteric bud branching; forebrain anterior/posterior pattern formation; spinal cord association neuron differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; negative regulation of cell-cell adhesion; positive regulation of protein amino acid phosphorylation; negative regulation of transforming growth factor beta receptor signaling pathway; Spemann organizer formation

Disease: Bone Mineral Density Quantitative Trait Locus 16; Osteogenesis Imperfecta, Type Xv