WBP11 sirna

WBP11 siRNA (Human)

Cat. Num. AAA8207925

• Gene Names: WBP11; NPWBP; SIPP1; WBP-11; PPP1R165
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: WBP11; WBP11 siRNA (Human); NPWBP; SIPP1; SNP70; WW domain-binding protein 11; WBP-11; Npw38-binding protein; NpwBP; SH3 domain-binding protein SNP70; Splicing factor that interacts with PQBP-1 and PP1; WBP11 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: WBP11 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 641

• Applicable Applications for WBP11 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human WBP11 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for WBP11 sirna

siRNA to inhibit WBP11 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 7706501
• NCBI GeneID: 51729
• NCBI Accession #: NP_057396.1
• NCBI GenBank Nucleotide #: NM_016312.2
• UniProt Accession #: Q9Y2W2; Q96AY8
• Molecular Weight: 69,998 Da
• NCBI Official Full Name: WW domain-binding protein 11
• NCBI Official Synonym Full Names: WW domain binding protein 11
• NCBI Official Symbol: WBP11
• NCBI Official Synonym Symbols: NPWBP; SIPP1; WBP-11; PPP1R165
• NCBI Protein Information: WW domain-binding protein 11
• UniProt Protein Name: WW domain-binding protein 11
• Protein Family: WW domain-binding protein
• UniProt Gene Name: WBP11
• UniProt Synonym Gene Names: NPWBP; SIPP1; SNP70; WBP-11; NpwBP
• UniProt Entry Name: WBP11_HUMAN

NCBI Description

This gene encodes a nuclear protein, which colocalizes with mRNA splicing factors and intermediate filament-containing perinuclear networks. This protein has 95% amino acid sequence identity to the mouse Wbp11 protein. It contains two proline-rich regions that bind to the WW domain of Npw38, a nuclear protein, and thus this protein is also called Npw38-binding protein NpwBP. The Npw38-NpwBP complex may function as a component of an mRNA factory in the nucleus. [provided by RefSeq, Jul 2008]

Uniprot Description

WBP11: Activates pre-mRNA splicing. May inhibit PP1 phosphatase activity.

Protein type: Spliceosome; RNA processing; DNA-binding; Protein phosphatase, regulatory subunit

Chromosomal Location of Human Ortholog: 12p12.3

Cellular Component: nucleoplasm; cytoplasm; nuclear speck; nucleus

Molecular Function: protein binding; protein phosphatase type 1 regulator activity; WW domain binding; single-stranded DNA binding

Biological Process: regulation of catalytic activity; RNA splicing; mRNA processing; rRNA processing