WAS sirna

WAS siRNA (Human)

Cat. Num. AAA8211361

• Gene Names: WAS; THC; IMD2; SCNX; THC1; WASP; WASPA
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: WAS; WAS siRNA (Human); IMD2; Wiskott-Aldrich syndrome protein; WASp; WAS sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: WAS siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 502

• Applicable Applications for WAS sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human WAS gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for WAS sirna

siRNA to inhibit WAS expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 4507909
• NCBI GeneID: 7454
• NCBI Accession #: NP_000368.1
• NCBI GenBank Nucleotide #: NM_000377.2
• UniProt Accession #: P42768; Q9BU11; Q9UNJ9
• Molecular Weight: 52,913 Da
• NCBI Official Full Name: wiskott-Aldrich syndrome protein
• NCBI Official Synonym Full Names: Wiskott-Aldrich syndrome
• NCBI Official Symbol: WAS
• NCBI Official Synonym Symbols: THC; IMD2; SCNX; THC1; WASP; WASPA
• NCBI Protein Information: wiskott-Aldrich syndrome protein
• UniProt Protein Name: Wiskott-Aldrich syndrome protein
• Protein Family: Waspkinin
• UniProt Gene Name: WAS
• UniProt Synonym Gene Names: IMD2; WASp
• UniProt Entry Name: WASP_HUMAN

NCBI Description

The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Wiskott-Aldrich syndrome is a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia, and is caused by mutations in the WAS gene. The WAS gene product is a cytoplasmic protein, expressed exclusively in hematopoietic cells, which show signalling and cytoskeletal abnormalities in WAS patients. A transcript variant arising as a result of alternative promoter usage, and containing a different 5' UTR sequence, has been described, however, its full-length nature is not known. [provided by RefSeq, Jul 2008]

Uniprot Description

WASP: a member of the Wiskott-Aldrich syndrome (WAS) family of proteins. A cytoplasmic protein expressed exclusively in hematopoietic cells. Transduces signals from surface receptors to the actin cytoskeleton. Associates with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Mutated in Wiskott-Aldrich syndrome, a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: Xp11.4-p11.21

Cellular Component: vesicle membrane; intercellular junction; cytosol; actin cytoskeleton

Molecular Function: identical protein binding; protein binding; phospholipase binding; actin binding; SH3 domain binding; protein kinase binding

Biological Process: epidermis development; T cell activation; actin filament-based movement; defense response; endosome transport; T cell receptor signaling pathway; actin filament polymerization; regulation of catalytic activity; actin polymerization and/or depolymerization; innate immune response; protein complex assembly; immune response; blood coagulation

Disease: Thrombocytopenia 1; Neutropenia, Severe Congenital, X-linked