WARS sirna

WARS siRNA (Human)

Cat. Num. AAA8229144

• Gene Names: WARS; IFI53; IFP53; GAMMA-2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: WARS; WARS siRNA (Human); IFI53; WRS; Tryptophan--tRNA ligase cytoplasmic; Interferon-induced protein 53; IFP53; Tryptophanyl-tRNA synthetase; TrpRS; hWRS; WARS sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: WARS siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 471

• Applicable Applications for WARS sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human WARS gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for WARS sirna

siRNA to inhibit WARS expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 47419914
• NCBI GeneID: 7453
• NCBI Accession #: NP_004175.2
• NCBI GenBank Nucleotide #: NM_004184.3
• UniProt Accession #: P23381; P78535; Q502Y0; Q53XB6; Q9UDI5; Q9UDL3; A6NGN1; A6NID3
• Molecular Weight: 48,852 Da
• NCBI Official Full Name: tryptophan--tRNA ligase, cytoplasmic isoform a
• NCBI Official Synonym Full Names: tryptophanyl-tRNA synthetase
• NCBI Official Symbol: WARS
• NCBI Official Synonym Symbols: IFI53; IFP53; GAMMA-2
• NCBI Protein Information: tryptophan--tRNA ligase, cytoplasmic
• UniProt Protein Name: Tryptophan--tRNA ligase, cytoplasmic
• Protein Family: Tryptophan--tRNA ligase
• UniProt Gene Name: WARS
• UniProt Synonym Gene Names: IFI53; WRS; IFP53; TrpRS; hWRS
• UniProt Entry Name: SYWC_HUMAN

NCBI Description

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

WARS: Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression. Belongs to the class-I aminoacyl-tRNA synthetase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Translation; EC 6.1.1.2; Amino Acid Metabolism - tryptophan; Ligase

Chromosomal Location of Human Ortholog: 14q32.31

Cellular Component: cytoplasm; nucleus; cytosol

Molecular Function: protein binding; tryptophan-tRNA ligase activity; ATP binding

Biological Process: negative regulation of cell proliferation; tryptophanyl-tRNA aminoacylation; tRNA aminoacylation for protein translation; translation; gene expression; angiogenesis; regulation of angiogenesis