VSX1 sirna

VSX1 siRNA (Human)

Cat. Num. AAA8204465

• Gene Names: VSX1; PPD; KTCN; PPCD; RINX; KTCN1; PPCD1; CAASDS
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: VSX1; VSX1 siRNA (Human); RINX; Visual system homeobox 1; Homeodomain protein RINX; Retinal inner nuclear layer homeobox protein; Transcription factor VSX1; VSX1 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: VSX1 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 236

• Applicable Applications for VSX1 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human VSX1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for VSX1 sirna

siRNA to inhibit VSX1 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 372266239
• NCBI GeneID: 30813
• NCBI Accession #: NP_001243200.1
• NCBI GenBank Nucleotide #: NM_001256271.1
• UniProt Accession #: Q9NZR4; Q0GM60; Q0GM61; Q0GM62; Q0GM63; Q0GM64; Q0GM65; Q5TF40; Q5TF41; Q9HCU3; B9EGJ4; D1MF28
• Molecular Weight: 32,054 Da
• NCBI Official Full Name: visual system homeobox 1 isoform c
• NCBI Official Synonym Full Names: visual system homeobox 1
• NCBI Official Symbol: VSX1
• NCBI Official Synonym Symbols: PPD; KTCN; PPCD; RINX; KTCN1; PPCD1; CAASDS
• NCBI Protein Information: visual system homeobox 1
• UniProt Protein Name: Visual system homeobox 1
• Protein Family: Visual system homeobox
• UniProt Gene Name: VSX1
• UniProt Synonym Gene Names: RINX
• UniProt Entry Name: VSX1_HUMAN

NCBI Description

The protein encoded by this gene contains a paired-like homeodomain and binds to the core of the locus control region of the red/green visual pigment gene cluster. The encoded protein may regulate expression of the cone opsin genes early in development. Mutations in this gene can cause posterior polymorphous corneal dystrophy and keratoconus. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

VSX1: Binds to the 37-bp core of the locus control region (LCR) of the red/green visual pigment gene cluster. May regulate the activity of the LCR and the cone opsin genes at earlier stages of development. Defects in VSX1 are a cause of posterior polymorphous corneal dystrophy type 1 (PPCD1). PPCD1 is a slowly progressive hereditary disorder of the corneal endothelium that leads to a variable degree of visual impairment usually in adulthood. Defects in VSX1 are a cause of keratoconus type 1 (KTCN1). Keratoconus type 1 is a frequent corneal dystrophy with an incidence that varies from 50 to 230 per 100'000. The cornea assumes a conical shape as a result of a progressive non- inflammatory thinning of the corneal stroma. Keratoconus is most often an isolated sporadic condition with cases of autosomal dominant and autosomal recessive transmission. Defects in VSX1 are the cause of craniofacial anomalies and anterior segment dysgenesis syndrome (CAASDS). CAASDS is a disorder with extremely variable expressivity. Clinical features include wide interpupillary distance, abnormal corneal endothelium, unusual pinnae, partially to completely empty sella turcica, posterior fossa cyst, anterior encephalocele, and/or hydrocephalus. Belongs to the paired homeobox family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Cell development/differentiation

Chromosomal Location of Human Ortholog: 20p11.21

Cellular Component: nucleus

Molecular Function: sequence-specific DNA binding; transcription factor activity

Biological Process: regulation of transcription, DNA-dependent; visual perception; transcription, DNA-dependent; response to stimulus; neuron maturation; retinal bipolar neuron differentiation

Disease: Corneal Dystrophy, Posterior Polymorphous, 1; Keratoconus 1; Craniofacial Anomalies And Anterior Segment Dysgenesis Syndrome