VAMP7 sirna

VAMP7 siRNA (Human)

Cat. Num. AAA8207437

• Gene Names: VAMP7; SYBL1; TIVAMP; VAMP-7; TI-VAMP
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: VAMP7; VAMP7 siRNA (Human); SYBL1; Vesicle-associated membrane protein 7; VAMP-7; Synaptobrevin-like protein 1; Tetanus-insensitive VAMP; Ti-VAMP; VAMP7 sirna

• Host: Synthetic
• Reactivity: Human
• Specificity: VAMP7 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 179

• Applicable Applications for VAMP7 sirna: RNA Interference (RNAi)
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human VAMP7 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for VAMP7 sirna

siRNA to inhibit VAMP7 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 223468574
• NCBI GeneID: 6845
• NCBI Accession #: NP_001138621.1
• NCBI GenBank Nucleotide #: NM_001145149.2
• UniProt Accession #: P51809; Q53GY7; Q7Z409; Q9H4A7
• Molecular Weight: 20,121 Da
• NCBI Official Full Name: vesicle-associated membrane protein 7 isoform 2
• NCBI Official Synonym Full Names: vesicle-associated membrane protein 7
• NCBI Official Symbol: VAMP7
• NCBI Official Synonym Symbols: SYBL1; TIVAMP; VAMP-7; TI-VAMP
• NCBI Protein Information: vesicle-associated membrane protein 7
• UniProt Protein Name: Vesicle-associated membrane protein 7
• Protein Family: Vesicle-associated membrane protein
• UniProt Gene Name: VAMP7
• UniProt Synonym Gene Names: SYBL1; VAMP-7; Ti-VAMP
• UniProt Entry Name: VAMP7_HUMAN

NCBI Description

This gene encodes a transmembrane protein that is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) family. The encoded protein localizes to late endosomes and lysosomes and is involved in the fusion of transport vesicles to their target membranes. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Jun 2010]

Uniprot Description

VAMP7: Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation. Belongs to the synaptobrevin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xq28 and Yq12

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; neuron projection; cell surface; intracellular membrane-bound organelle; late endosome membrane; lysosomal membrane; secretory granule membrane; integral to membrane; trans-Golgi network; phagocytic vesicle; pseudopodium; secretory granule; SNARE complex; phagocytic vesicle membrane; membrane; lamellipodium; perinuclear region of cytoplasm; apical part of cell; cytoplasm; plasma membrane; synapse; cell junction

Molecular Function: SNAP receptor activity; SNARE binding; protein binding; syntaxin binding

Biological Process: ER to Golgi vesicle-mediated transport; exocytosis; autophagic vacuole fusion; natural killer cell degranulation; neutrophil degranulation; endocytosis; vesicle fusion with Golgi apparatus; calcium ion-dependent exocytosis; vesicle fusion; vesicle-mediated transport; Golgi to plasma membrane protein transport; eosinophil degranulation; endosome to lysosome transport; triacylglycerol transport; post-Golgi vesicle-mediated transport; phagocytosis, engulfment